Overview

  • Product name
    Anti-PDPK1 (phospho Y9) antibody
    See all PDPK1 primary antibodies
  • Description
    Rabbit polyclonal to PDPK1 (phospho Y9)
  • Specificity
    ab45375 detects a 65 kDa protein corresponding to the apparent molecular mass of PDPK1.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: RTTSQLYPDAVPIQS, corresponding to amino acids 3-16 of Human PDPK1

  • Positive control
    • A431 cells treated with EGF, IGF-1, or pervanadate and adult mouse brain.

Properties

Applications

Our Abpromise guarantee covers the use of ab45375 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 63 kDa).
ELISA 1/2000.

Target

  • Function
    Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.
  • Tissue specificity
    Appears to be expressed ubiquitously.
  • Sequence similarities
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDK1 subfamily.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine and serine/threonine. Phosphorylation on Ser-241 in the activation loop is required for full activity. PDK1 itself can autophosphorylate Ser-241, leading to its own activation.
  • Cellular localization
    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation. Tyrosine phosphorylation seems to occur only at the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 phosphoinositide dependent protein kinase 1 antibody
    • 3-phosphoinositide-dependent protein kinase 1 antibody
    • hPDK 1 antibody
    • hPDK1 antibody
    • MGC20087 antibody
    • MGC35290 antibody
    • OTTHUMP00000159109 antibody
    • OTTHUMP00000159110 antibody
    • OTTHUMP00000174525 antibody
    • PDK1 antibody
    • Pdpk1 antibody
    • PDPK1_HUMAN antibody
    • PDPK2 antibody
    • PDPK2P antibody
    • PkB kinase antibody
    • PkB kinase like gene 1 antibody
    • PkB like 1 antibody
    • PRO0461 antibody
    • Protein kinase antibody
    see all

References

ab45375 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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