Anti-AS3 antibody (ab70298)
Key features and details
- Rabbit polyclonal to AS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-AS3 antibody
See all AS3 primary antibodies -
Description
Rabbit polyclonal to AS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T and NIH3T3 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70298 was affinity purified using an epitope specific to AS3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair. Plays a role in androgen-induced proliferative arrest in prostate cells. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the PDS5 family.
Contains 3 A.T hook DNA-binding domains.
Contains 1 HEAT repeat. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 23047 Human
- Entrez Gene: 100710 Mouse
- Omim: 605333 Human
- SwissProt: Q9NTI5 Human
- SwissProt: Q4VA53 Mouse
- Unigene: 744901 Human
- Unigene: 32959 Mouse
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Alternative names
- AI646570 antibody
- Androgen induced proliferation inhibitor antibody
- Androgen-induced proliferation inhibitor antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma (left) and prostate carcinoma (right) tissues labelling AS3 with ab70298 at 1/200 (1µg/ml) and 1/80 (2.5µg/ml). Detection: DAB and DyLight® 594-conjugated goat anti-rabbit IgG (1/100).
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All lanes : Anti-AS3 antibody (ab70298) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 165 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds -
Western Blot showing ab70298 at 0.1µg/ml staining AS3 in HeLa whole cell lysates immunoprecipitated using ab70298 at 3µg/mg lysate (1 mg/IP loaded/lane).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab70298 has been referenced in 2 publications.
- Shiimori M et al. Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. iScience 24:102660 (2021). PubMed: 34169237
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942