• Product name
  • Description
    Rabbit polyclonal to PDS5B
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between C terminanl residues 1400 and 1447 of human PDS5B

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab70299 was affinity purified using an epitope specific to PDS5B immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 165 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Relevance
    PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
  • Cellular localization
  • Database links
  • Alternative names
    • AI646570 antibody
    • Androgen induced proliferation inhibitor antibody
    • Androgen-induced prostate proliferative shutoff associated protein AS3 antibody
    • APRIN antibody
    • AS3 antibody
    • AW212954 antibody
    • CG008 antibody
    • FLJ23236 antibody
    • KIAA0979 antibody
    • mKIAA0979 antibody
    • PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) antibody
    • Pds5b antibody
    • RP1 267P19.1 antibody
    • Sister chromatid cohesion protein PDS5 homolog B antibody
    see all

Anti-PDS5B antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human laryngeal squamous cell carcinoma tissue labelling PDS5B with ab70299 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PDS5B antibody (ab70299) at 0.04 µg/ml

    Lane 1 : Jurkat cell lysate at 50 µg
    Lane 2 : Jurkat cell lysate at 15 µg
    Lane 3 : Jurkat cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : NIH3T3 cell lysate at 50 µg
    Lane 6 : 293T cell lysate at 50 µg
    Lane 7 : NIH3T3 cell lysate at 50 µg

    Predicted band size : 165 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Detection: Chemiluminescence with exposure times of 10 seconds (lanes 1-5) and 3 minutes (lanes 6-7).
  • Western Blot showing ab70299 at 1µg/ml staining PDS5B in HeLa whole cell lysates immunoprecipitated using Lane 1: ab70298 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 2: ab70299 at 3µg/mg lysate (1 mg/IP; 1/4 of IP loaded/lane). Lane 3: control IgG was used for immunoprecipitation. Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-PDS5B antibody (ab70299)

ab70299 has not yet been referenced specifically in any publications.

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