Purification notesab84918 was affinity purified using an epitope specific to Pds5B immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals
1.0 mg of IgG.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevancePDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
ab84918, at a 1/100 dilution, staining PDS5B in formalin fixed, paraffin embedded human prostate carcinoma by Immunohistochemistry. Detection: a Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody at a dilution of 1/100.