Overview

  • Product name
  • Description
    Rabbit polyclonal to PDS5B
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1200 and 1250 of human PDS5B (CAB69911.1).

  • Positive control
    • Human controls: Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell Carcinoma, Testicular Seminoma Mouse control: Squamous Cell Carcinoma

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84918 was affinity purified using an epitope specific to Pds5B immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining.

    Likely to work with frozen sections.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      PDS5B, also known as APRIN (Androgen induced proliferation inhibitor) plays a role in androgen-induced proliferative arrest in prostate cells and is required for maintenance of sister chromatid cohesion during mitosis.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • AI646570 antibody
      • Androgen induced proliferation inhibitor antibody
      • Androgen-induced prostate proliferative shutoff associated protein AS3 antibody
      • APRIN antibody
      • AS3 antibody
      • AW212954 antibody
      • CG008 antibody
      • FLJ23236 antibody
      • KIAA0979 antibody
      • mKIAA0979 antibody
      • PDS5, regulator of cohesion maintenance, homolog B (S. cerevisiae) antibody
      • Pds5b antibody
      • RP1 267P19.1 antibody
      • Sister chromatid cohesion protein PDS5 homolog B antibody
      see all

    Images

    • ab84918, at a 1/100 dilution, staining PDS5B in formalin fixed, paraffin embedded human prostate carcinoma by Immunohistochemistry. Detection: a Red fluorescent goat anti-rabbit IgG highly cross adsorbed antibody at a dilution of 1/100.
    • ab84918, at a 1/250 dilution, staining PDS5B in formalin fixed, paraffin embedded human prostate carcinoma by Immunohistochemistry, using DAB staining.
    • ab84918, at a 1/250 dilution, staining PDS5B in formalin fixed, paraffin embedded mouse squamous cell carcinoma by Immunohistochemistry, using DAB staining.

    References

    This product has been referenced in:
    • Brieño-Enríquez MA  et al. Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1?-WAPL Axis in the Mouse. Cell Rep 17:977-986 (2016). Read more (PubMed: 27760328) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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