Anti-PDX1 antibody (ab227586)
Key features and details
- Rabbit polyclonal to PDX1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PDX1 antibody
See all PDX1 primary antibodies -
Description
Rabbit polyclonal to PDX1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Dog, Pig -
Immunogen
Synthetic peptide within Human PDX1 (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P52945 -
Positive control
- WB: Jurkat, Raji, NCI-H929 and AsPC-1 whole cell extracts; Mouse liver and pancreas liver tissue extracts; Rat liver tissue extract. IHC-P: Rat pancreas tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 31 kDa.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 31 kDa. |
Target
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Function
Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. -
Tissue specificity
Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). -
Involvement in disease
Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease. -
Sequence similarities
Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Contains 1 homeobox DNA-binding domain. -
Domain
The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y). -
Post-translational
modificationsPhosphorylated by the SAPK2 pathway at high intracellular glucose concentration. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 493994 Dog
- Entrez Gene: 3651 Human
- Entrez Gene: 18609 Mouse
- Entrez Gene: 397288 Pig
- Entrez Gene: 29535 Rat
- Omim: 600733 Human
- SwissProt: P52945 Human
- SwissProt: P52946 Mouse
see all -
Alternative names
- Glucose sensitive factor antibody
- Glucose-sensitive factor antibody
- GSF antibody
see all
Images
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Paraffin-embedded rat pancreas tissue stained for PDX1 using ab227586 at 1/500 dilution in immunohistochemical analysis.
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All lanes : Anti-PDX1 antibody (ab227586) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Mouse pancreas tissue extract at 50 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Anti-PDX1 antibody (ab227586) at 1/1000 dilution + AsPC-1 whole cell extract at 30 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Rat liver tissue extract at 50 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
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Anti-PDX1 antibody (ab227586) at 1/1000 dilution + Mouse liver tissue extract at 50 µg
Predicted band size: 31 kDa12% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab227586 has been referenced in 1 publication.
- Gao D et al. The Roles of Different Multigene Combinations of Pdx1, Ngn3, Sox9, Pax4, and Nkx2.2 in the Reprogramming of Canine ADSCs Into IPCs. Cell Transplant 31:9636897221081483 (2022). PubMed: 35236160