Overview

  • Product name
  • Description
    Rabbit polyclonal to PDX1
  • Tested applications
    Suitable for: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
  • Immunogen

    Recombinant fusion protein containing N-Terminal region of mouse Pdx1

Properties

Applications

Our Abpromise guarantee covers the use of ab47267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/5000.
WB 1/3000 - 1/5000. Predicted molecular weight: 31 kDa.
ICC/IF 1/2000.

See ICC-specific protocol in protocol tab.

IHC-P Use at an assay dependent concentration.

See IHC-specific protocol in protocol tab.

Target

  • Function
    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease
    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain
    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications
    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Images

  • Immunohistochemical analysis of murine pancreatic islets, staining PDX1 (red) with ab47267.

    Tissue was fixed in formalin, permeablized with 0.25% Triton X-100, and blocked in blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody overnight at 4°C and a fluorescent-conjugated anti-rabbit IgG was used as the secondary antibody.
  • ab47267 staining PDX1 in rat pancreatic tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. 

    See Abreview

  • ab47267 staining PDX1 in embryonic (day 13.5) mouse pancreas tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 10% serum for 30 minutes at 220C was performed. The sample was incubated with primary antibody (1/5000) in 10% serum for 16 hours in 220C. A biotin-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/250 as secondary antibody. A biotin/avidin/streptavidin linked to HRP system has been used.

    See Abreview

  • Immunostaining of islet-epithelial clusters from human foetal pancreata with ab47267 (green). Nuclei are visualised with DAPI (blue).
  • ab47267 staining PDX1 in human appendix tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxide block and 10 minutes for protein block). The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary. 

References

This product has been referenced in:
  • Qiu G  et al. Formononetin exhibits anti-hyperglycemic activity in alloxan-induced type 1 diabetic mice. Exp Biol Med (Maywood) 242:223-230 (2017). Read more (PubMed: 27412955) »
  • Wang W  et al. PDX1 and ISL1 differentially coordinate with epigenetic modifications to regulate insulin gene expression in varied glucose concentrations. Mol Cell Endocrinol 428:38-48 (2016). Read more (PubMed: 26994512) »

See all 35 Publications for this product

Customer reviews and Q&As

Thank you for contacting Abcam.

Currently we only sell this product in 100ul sizes, but if you are interested in placing a large bulk order (greater to 10 units) then we could look into a custom order.

If this is something that you wo...

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH=9.0
Permeabilization
Yes - PBS with 3% Triton-100
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 22 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® iPSC Diff Day 5)
Permeabilization
No
Specification
iCell® iPSC Diff Day 5
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

Submitted Sep 17 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (H9 cell line upon differentiation)
Specification
H9 cell line upon differentiation
Permeabilization
Yes - 0.01% Triton X100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 22 2014

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody. I appreciate your patience, it has taken some time to obtain the testing protocol for you.

I would lik...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human embryonic stem cell derived-pancreatic endoc)
Specification
human embryonic stem cell derived-pancreatic endoc
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Youngjin Kim

Verified customer

Submitted Oct 25 2012

Thank you for contacting us.

The response to the inquiry you found contained proprietary information and was therefore not published. However, we did find that the immunogen used to create this product had a sequence similarity is 64% to c...

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Thank you very much for contacting us.

We currently do not have the immunogen sequence of ab47267 available, but I have contacted the originator for the information you requested, and I am awaiting a reply. I will forward to you the sequence ...

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Thank you for contacting Abcam. We have not quantitated the antibody, so I am not able to provide a concentration. Sorry for not being able to specifically answer the question, but please let me know if I can be of any other assistance.

I have issued a free of charge replacement with the order number ****. This is for rabbit anti-PDX1, ab47267, to replace ab47308. The fixation is with 4% PFA for 5 minutes, followed by permeabilization with Triton X-100. The full protocol is below...

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