Overview

  • Product name
  • Description
    Guinea pig polyclonal to PDX1
  • Tested applications
    Suitable for: IHC-FoFr, IHC-Fr, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fusion protein containing N-terminal sequence from mouse PDX1

Properties

Applications

Our Abpromise guarantee covers the use of ab47308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 20170518
IHC-Fr 1/200.
IHC-P Use at an assay dependent dilution.
ICC/IF 1/10000.

Target

  • Function
    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease
    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain
    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications
    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Anti-PDX1 antibody images

  • Immunohistochemical analysis of developing murine pancreas taken from wild-type (+/+) and SEL1L-null mice (-/-). PDX1 (green) was stained with ab47308.

    Tissue was fixed with paraformaldehyde before freezing and sectioning. Sections were permeabilized with 0.2% Triton X-100 for 20 min and blocked with 5% normal donkey serum and 1% BSA in PBS at room temperature for at least 1 hour. Samples were incubated with primary antibody (1/100 in 1% BSA in PBS) overnight at 4°C. A Cy2®-conjugated donkey anti-guinea pig IgG (1/500) was used as the secondary antibody.
  • Immunohistochemical analysis of paraformaldehyde-fixed,0.1 % Triton X-100 permeabilized mouse pancreas tissue labelling PDX1 with ab47308 at 1/200 dilution,followed by Goat Anti-guinea pig IgG conjugation Alexa Fluor®488 secondary antibody at 1/200 dilution.

    See Abreview

  • Immunostaining of islets from 4 week-old mice with ab47308 guinea pig anti-PDX1 (red) and an anti-insulin antibody (green).

References for Anti-PDX1 antibody (ab47308)

This product has been referenced in:
  • Phelps EA  et al. Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation. Sci Rep 7:45961 (2017). Read more (PubMed: 28401888) »
  • Konno M  et al. Embryonic MicroRNA-369 Controls Metabolic Splicing Factors and Urges Cellular Reprograming. PLoS One 10:e0132789 (2015). ICC/IF ; Mouse . Read more (PubMed: 26176628) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6.0
Sample
Mouse Tissue sections (pancreas)
Specification
pancreas
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH6.0
Sample
Human Tissue sections (pancreas)
Specification
pancreas
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 26 2013

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8 % gel)
Sample
Human Cell lysate - whole cell (Pancreatic progenitors from hPSCs)
Specification
Pancreatic progenitors from hPSCs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Sep 10 2013

Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital information for monit...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Apr 16 2012

Thank you for contacting us.

PDX1 will be expressed in the nucleus ofpancreatic islet and intercalated duct cellsin adult mice. I hope this helps, please let me know if you need any additional information.

I have issued a free of charge replacement with the order number ****. This is for rabbit anti-PDX1, ab47267, to replace ab47308. The fixation is with 4% PFA for 5 minutes, followed by permeabilization with Triton X-100. The full protocol is below...

Read More

I assume you have the antibody ab47267, as that is the only rabbit anti-PDX1 in our catalogue that has been tested for ICC. We do not have samples of any of our antibodies but I can send a full vial as a replacement, perhaps from a lot different from w...

Read More

I am sorry to read that this is still not working for you. My guess is that the fixation is still too long at 2 hours, even with 4% vs. 10% PFA, or that there is lot-to-lot variability with regards to compatibility with formaldeyhe-based fixation. ...

Read More

Following up my previous message, the laboratory and I think the problem may be the 10% PFA fixation for 10 minutes at room temperature. The lab used 4% PFA and fixed for 2 hours at 4C. I think the fixation time could be made shorter than two ho...

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up