Overview

  • Product name
    Anti-PDX1 antibody [OTI2A12]
    See all PDX1 primary antibodies
  • Description
    Mouse monoclonal [OTI2A12] to PDX1
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-283 of human PDX1 expressed in E. coli.

  • Positive control
    • IHC-P: pancreas; carcinoma of pancreas WB: HEK293T cells transfected with PDX1; lysate from 293T cells transfected with PDX1 ICC/IF: HeLa cells transiently transfected by PDX1; HeLa cells
  • General notes

    The clone number has been updated from 2A12 to OTI2A12, both clone numbers name the same clone.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

Properties

Applications

Our Abpromise guarantee covers the use of ab84987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Predicted molecular weight: 31 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • Tissue specificity
    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • Involvement in disease
    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • Sequence similarities
    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • Domain
    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • Post-translational
    modifications
    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

Images

  • ab84987, staining PDX1 in paraffin embedded human pancreas carcinoma tissue by Immunohistochemistry.
  • ab84987, staining PDX1 in paraffin embedded human pancreas by Immunohistochemistry.
  • Anti-PDX1 antibody [OTI2A12] (ab84987) at 1/20000 dilution + lysate from HEK293T cells transfected with human PDX1 expression vector

    Predicted band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)
  • All lanes : Anti-PDX1 antibody [OTI2A12] (ab84987)

    Lane 1 : lysate from HEK293T cells transfected with an empty expression vector
    Lane 2 : lysate from HEK293T cells transfected with a human PDX1 expression vector

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 31 kDa
    Observed band size : 31 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200033)
  • ab84987, staining PDX1 by Immunofluoresence in HeLa cells transiently transfected with a vector expressing PDX1.

References

This product has been referenced in:
  • Jafarian A  et al. The Generation of Insulin Producing Cells from Human Mesenchymal Stem Cells by MiR-375 and Anti-MiR-9. PLoS One 10:e0128650 (2015). IF . Read more (PubMed: 26047014) »
  • Allahverdi A  et al. Differentiation of Human Mesenchymal Stem Cells into Insulin Producing Cells by Using A Lentiviral Vector Carrying PDX1. Cell J 17:231-42 (2015). WB . Read more (PubMed: 26199902) »

See all 3 Publications for this product

Customer reviews and Q&As

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We currently do not have the immunogen sequence of ab47267 available, but I have contacted the originator for the information you requested, and I am awaiting a reply. I will forward to you the sequence ...

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