Overview

  • Product nameAnti-PDXK.1 antibody [5H5]
    See all PDXK.1 primary antibodies
  • Description
    Mouse monoclonal [5H5] to PDXK.1
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human PDXK.1 produced in HEK293 cells (NP_003672.1).

  • Positive control
    • HEK293T cell lysate transfected with pCMV6-ENTRY PDXK.1 cDNA; COS7 cells transiently transfected by pCMV6-ENTRY PDXK.1; HEK293T cells transfected with either PDXK.1 overexpress plasmid.
  • General notesDilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

Properties

Applications

Our Abpromise guarantee covers the use of ab119051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 35 kDa.
Flow Cyt 1/100. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100.

Target

  • FunctionRequired for synthesis of pyridoxal-5-phosphate from vitamin B6.
  • Tissue specificityUbiquitous. Isoform 3 is detected in adult testis and spermatozoa.
  • Sequence similaritiesBelongs to the pyridoxine kinase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • pdxK antibody
    • PDXK_HUMAN antibody
    • PKH antibody
    • PNK antibody
    • Pyridoxal kinase antibody
    • Pyridoxamine kinase antibody
    • Pyridoxine kinase antibody
    • Pyridoxine kinase; antibody
    • Vitamin B6 kinase antibody
    see all

Anti-PDXK.1 antibody [5H5] images

  • All lanes : Anti-PDXK.1 antibody [5H5] (ab119051) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY PDXK.1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 35 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200975)
  • ab119051 at 1/100 dilution staining PDXK.1 in COS7 cells transiently transfected with pCMV6-ENTRY PDXK.1 by Immunofluorescence.
  • ab119051 at 1/100 dilution staining PDXK.1 in HEK293T cells transfected with either pCMV6-ENTRY PDXK.1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by flow cytometry.

References for Anti-PDXK.1 antibody [5H5] (ab119051)

ab119051 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"