Overview

  • Product nameAnti-PDXK.1 antibody [OTI5H5]
    See all PDXK.1 primary antibodies
  • Description
    Mouse monoclonal [OTI5H5] to PDXK.1
  • Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length Human PDXK.1 produced in HEK293 cells (NP_003672.1).

  • Positive control
    • WB: HepG2, HeLa, SVT2, A%49, COS7, Jurkat and MCF7 cell lysates. IHC-P: Human ovary adenocarcinoma, pancreas, endometrium, endometrium adenocarcinoma, bladder carcinoma and lymph nodes. ICC/IF: COS7 cells transiently transfected by pCMV6-ENTRY PDXK. Flow Cyt: Transfected HEK293T cells.
  • General notes

    Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.

    The clone number has been updated from 5H5 to OTI5H5, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab119051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 35 kDa.
Flow Cyt 1/100.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF 1/100.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRequired for synthesis of pyridoxal-5-phosphate from vitamin B6.
  • Tissue specificityUbiquitous. Isoform 3 is detected in adult testis and spermatozoa.
  • Sequence similaritiesBelongs to the pyridoxine kinase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • pdxK antibody
    • PDXK_HUMAN antibody
    • PKH antibody
    • PNK antibody
    • Pyridoxal kinase antibody
    • Pyridoxamine kinase antibody
    • Pyridoxine kinase antibody
    • Pyridoxine kinase; antibody
    • Vitamin B6 kinase antibody
    see all

Anti-PDXK.1 antibody [OTI5H5] images

  • All lanes : Anti-PDXK.1 antibody [OTI5H5] (ab119051) at 1/200 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 35 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adenocarcinoma of human ovary tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of adenocarcinoma of human endometrium tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of carcinoma of human bladder tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node tissue sections labelling PDXK.1 with ab119051. Heat-induced antigen retrieval was performed using 10mM citric buffer, pH 6.0 at 100°C for 10 minutes.

  • ab119051 at 1/100 dilution staining PDXK.1 in COS7 cells transiently transfected with pCMV6-ENTRY PDXK.1 by Immunofluorescence.
  • ab119051 at 1/100 dilution staining PDXK.1 in HEK293T cells transfected with either pCMV6-ENTRY PDXK.1 overexpress plasmid(Red) or empty vector control plasmid(Blue) by flow cytometry.
  • All lanes : Anti-PDXK.1 antibody [OTI5H5] (ab119051) at 1/2000 dilution

    Lane 1 : HEK293T lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK293T lysate transfected with pCMV6-ENTRY PDXK.1 cDNA

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 35 kDa
    HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC200975)

References for Anti-PDXK.1 antibody [OTI5H5] (ab119051)

ab119051 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab119051.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"