Overview

  • Product nameAnti-PDZK1 antibody
    See all PDZK1 primary antibodies
  • Description
    Rabbit polyclonal to PDZK1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Cow, Chinese Hamster
  • Immunogen

    Recombinant fragment: MTSTFNPREC KLSKQEGQNY GFFLRIEKDT EGHLVRVVEK CSPAEKAGLQ DGDRVLRING VFVDKEEHMQ VVDLVRKSGN SVTLLVLDGD SYEKAV, corresponding to amino acids 1-96 of Human PDZK1

  • Positive control
    • Human liver lysate; Human kidney tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

WB 1/250 - 1/500. Predicted molecular weight: 57 kDa.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.

Target

  • FunctionA scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
  • Tissue specificityExpression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas.
  • Sequence similaritiesBelongs to the NHER family.
    Contains 4 PDZ (DHR) domains.
  • DomainThe PDZ 2 and 3 domains seem to be involved in the interaction with SLC26A3.
    Interaction with the C-terminus of CFTR could be mediated through independent binding of PDZ 1, 3 and 4 domains.
    The PDZ 1 and 3 domains seem to be involved in the interaction with SLCO1A1.
    The PDZ 1 domain interacts with BCR.
  • Cellular localizationCytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700023D20Rik antibody
    • 2610507N21Rik antibody
    • 4921513F16Rik antibody
    • AI267131 antibody
    • AI314638 antibody
    • AL022680 antibody
    • C terminal linking and modulating protein antibody
    • CAP70 antibody
    • CFTR associated protein of 70 kDa antibody
    • CFTR associated protein, 70-KD antibody
    • CFTR-associated protein of 70 kDa antibody
    • CLAMP antibody
    • D3Ertd537e antibody
    • Dietary Pi-regulated RNA-1 antibody
    • Diphor-1 antibody
    • mPDZK1 antibody
    • Na(+)/H(+) exchange regulatory cofactor NHE-RF3 antibody
    • Na(+)/H(+) exchanger regulatory factor 3 antibody
    • Na/Pi cotransporter C-terminal-associated protein 1 antibody
    • Na/Pi cotransporter C-terminal-associated protein antibody
    • NaPi Cap1 antibody
    • NaPi-Cap1 antibody
    • NaPiCap1 antibody
    • NHERF 3 antibody
    • NHERF-3 antibody
    • NHERF3 antibody
    • NHRF3_HUMAN antibody
    • OTTHUMP00000015572 antibody
    • PDZ domain containing 1 antibody
    • PDZ domain containing protein 1 antibody
    • PDZ domain-containing protein 1 antibody
    • PDZD1 antibody
    • PDZK1 antibody
    • Sodium hydrogen exchanger regulatory factor 3 antibody
    • Sodium-hydrogen exchanger regulatory factor 3 antibody
    see all

Anti-PDZK1 antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and vesicles. Recommended concentration of ab121248 1-4 µg/ml. Cells treated with PFA/Triton X-100.


  • Predicted band size : 57 kDa
    Lane 1: Mouse liver tissue lysate
    Lane 2: Rat liver tissue lysate
  • All lanes : Anti-PDZK1 antibody (ab121248) at 1/250 dilution

    Lane 1 : Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
    Lane 2 : RT-4 cell lysate
    Lane 3 : U-251 MG cell lysate
    Lane 4 : RT-4 lysate
    Lane 5 : Liver lysate
    Lane 6 : Tonsil lysate


    Predicted band size : 57 kDa
  • Immunohistochemical staining of paraffin embedded Human kidney, using 1/150 ab121248, shows strong cytoplasmic positivity in cells in tubules.

References for Anti-PDZK1 antibody (ab121248)

ab121248 has not yet been referenced specifically in any publications.

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