Overview

  • Product nameAnti-PDZK1 antibody [EPR3751]
    See all PDZK1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3751] to PDZK1
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PDZK1.

  • Positive control
    • WB: Human fetal liver, fetal kidney and kidney tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab92491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
IP 1/50.
  • Application notesIs unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • FunctionA scaffold protein that connects plasma membrane proteins and regulatory components, regulating their surface expression in epithelial cells apical domains. May be involved in the coordination of a diverse range of regulatory processes for ion transport and second messenger cascades. In complex with SLC9A3R1, may cluster proteins that are functionally dependent in a mutual fashion and modulate the trafficking and the activity of the associated membrane proteins. May play a role in the cellular mechanisms associated with multidrug resistance through its interaction with ABCC2 and PDZK1IP1. May potentiate the CFTR chloride channel activity. May function to connect SCARB1 with the cellular machineries for intracellular cholesterol transport and/or metabolism. May be involved in the regulation of proximal tubular Na(+)-dependent inorganic phosphate cotransport therefore playing an important role in tubule function.
    • Tissue specificityExpression is limited to epithelial cells. Expressed in the kidney (brush border of proximal tubule), pancreas, liver, and small intestine. Expressed at a lower level in the adrenal cortex, testis and stomach. Overexpressed in breast, renal and lung carcinomas.
    • Sequence similaritiesBelongs to the NHER family.
      Contains 4 PDZ (DHR) domains.
    • DomainThe PDZ 2 and 3 domains seem to be involved in the interaction with SLC26A3.
      Interaction with the C-terminus of CFTR could be mediated through independent binding of PDZ 1, 3 and 4 domains.
      The PDZ 1 and 3 domains seem to be involved in the interaction with SLCO1A1.
      The PDZ 1 domain interacts with BCR.
    • Cellular localizationCytoplasm. Membrane. Associated with peripheral membranes. Localizes to the apical compartment of proximal tubular cells and to sinusoidal liver membranes.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1700023D20Rik antibody
      • 2610507N21Rik antibody
      • 4921513F16Rik antibody
      • AI267131 antibody
      • AI314638 antibody
      • AL022680 antibody
      • C terminal linking and modulating protein antibody
      • CAP70 antibody
      • CFTR associated protein of 70 kDa antibody
      • CFTR associated protein, 70-KD antibody
      • CFTR-associated protein of 70 kDa antibody
      • CLAMP antibody
      • D3Ertd537e antibody
      • Dietary Pi-regulated RNA-1 antibody
      • Diphor-1 antibody
      • mPDZK1 antibody
      • Na(+)/H(+) exchange regulatory cofactor NHE-RF3 antibody
      • Na(+)/H(+) exchanger regulatory factor 3 antibody
      • Na/Pi cotransporter C-terminal-associated protein 1 antibody
      • Na/Pi cotransporter C-terminal-associated protein antibody
      • NaPi Cap1 antibody
      • NaPi-Cap1 antibody
      • NaPiCap1 antibody
      • NHERF 3 antibody
      • NHERF-3 antibody
      • NHERF3 antibody
      • NHRF3_HUMAN antibody
      • OTTHUMP00000015572 antibody
      • PDZ domain containing 1 antibody
      • PDZ domain containing protein 1 antibody
      • PDZ domain-containing protein 1 antibody
      • PDZD1 antibody
      • PDZK1 antibody
      • Sodium hydrogen exchanger regulatory factor 3 antibody
      • Sodium-hydrogen exchanger regulatory factor 3 antibody
      see all

    Anti-PDZK1 antibody [EPR3751] images

    • All lanes : Anti-PDZK1 antibody [EPR3751] (ab92491) at 1/5000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 57 kDa
      Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-PDZK1 antibody [EPR3751] (ab92491) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : T47-D (human mammary gland ductal carcinoma) cell lysate
      Lane 4 : Caco-2 (human colorectal adenocarcinoma) cell lysate
      Lane 5 : HeLa (human cervix adenocarcinoma) cell lysate
      Lane 6 : MCF-7 (human breast carcinoma) cell lysate

      Lysates/proteins at 20 µg/ml per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 57 kDa


      Exposure time : 5 seconds

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    References for Anti-PDZK1 antibody [EPR3751] (ab92491)

    ab92491 has not yet been referenced specifically in any publications.

    Product Wall

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