Overview

  • Product nameAnti-PEA15 (phospho S116) antibody
    See all PEA15 primary antibodies
  • Description
    Rabbit polyclonal to PEA15 (phospho S116)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from a region of human PED/PEA-15 that contains serine 116.

  • Positive control
    • Cos celss transfected with PED cDNAand stimulated with 100ng/mL PMA;SK-BR-3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1.0mg/ml BSA. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from rabbit serum by epitope-specific affinity chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PED/PEA-15. The final product is generated by affinity chromatography using a PED/PEA-15-derived peptide that is phosphorylated at serine 116.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab18032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).

Target

  • FunctionBlocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
  • Tissue specificityUbiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
  • Sequence similaritiesContains 1 DED (death effector) domain.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
  • Cellular localizationCytoplasm. Associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 kDa phosphoprotein enriched in astrocytes antibody
    • Astrocytic phosphoprotein PEA 15 antibody
    • Astrocytic phosphoprotein PEA-15 antibody
    • Astrocytic phosphoprotein PEA15 antibody
    • HMAT 1 antibody
    • HMAT1 antibody
    • Homolog of mouse MAT 1 oncogene antibody
    • Homolog of mouse MAT1 oncogene antibody
    • HUMMAT 1H antibody
    • HUMMAT1H antibody
    • MAT 1 antibody
    • MAT 1H antibody
    • MAT1 antibody
    • MAT1H antibody
    • PEA 15 antibody
    • Pea15 antibody
    • PEA15 protein antibody
    • PEA15_HUMAN antibody
    • PED antibody
    • Phosphoprotein enriched in astrocytes 15 antibody
    • Phosphoprotein enriched in astrocytes 15kD antibody
    • Phosphoprotein enriched in diabetes antibody
    see all

References for Anti-PEA15 (phospho S116) antibody (ab18032)

This product has been referenced in:
  • Mielgo A  et al. Paclitaxel promotes a caspase 8-mediated apoptosis through death effector domain association with microtubules. Oncogene 28:3551-62 (2009). ICC/IF ; Human . Read more (PubMed: 19668227) »
  • Callejas-Valera JL  et al. E1a Gene Expression Blocks the ERK1/2 Signaling Pathway by Promoting Nuclear Localization and MKP Up-regulation: IMPLICATION IN v-H-Ras-INDUCED SENESCENCE. J Biol Chem 283:13450-8 (2008). WB . Read more (PubMed: 18316372) »

See all 2 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab18032.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"