Anti-PEA15 (phospho S116) antibody (ab59216)

Overview

  • Product name
    Anti-PEA15 (phospho S116) antibody
    See all PEA15 primary antibodies
  • Description
    Rabbit polyclonal to PEA15 (phospho S116)
  • Specificity
    ab59216 detects endogenous levels of PEA 15 only when phosphorylated at serine 116.
  • Tested applications
    Suitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PEA 15 (SwissProt Q15121) around the phosphorylation site of serine 116 (Q-P-SP-E-E).

  • Positive control
    • Human skeletal muscle tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab59216 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • Function
    Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.
  • Tissue specificity
    Ubiquitously expressed. Most abundant in tissues such as heart, brain, muscle and adipose tissue which utilize glucose as an energy source. Lower expression in glucose-producing tissues. Higher levels of expression are found in tissues from individuals with type 2 diabetes than in controls.
  • Sequence similarities
    Contains 1 DED (death effector) domain.
  • Post-translational
    modifications
    Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.
  • Cellular localization
    Cytoplasm. Associated with microtubules.
  • Information by UniProt
  • Database links
  • Alternative names
    • 15 kDa phosphoprotein enriched in astrocytes antibody
    • Astrocytic phosphoprotein PEA 15 antibody
    • Astrocytic phosphoprotein PEA-15 antibody
    • Astrocytic phosphoprotein PEA15 antibody
    • HMAT 1 antibody
    • HMAT1 antibody
    • Homolog of mouse MAT 1 oncogene antibody
    • Homolog of mouse MAT1 oncogene antibody
    • HUMMAT 1H antibody
    • HUMMAT1H antibody
    • MAT 1 antibody
    • MAT 1H antibody
    • MAT1 antibody
    • MAT1H antibody
    • PEA 15 antibody
    • Pea15 antibody
    • PEA15 protein antibody
    • PEA15_HUMAN antibody
    • PED antibody
    • Phosphoprotein enriched in astrocytes 15 antibody
    • Phosphoprotein enriched in astrocytes 15kD antibody
    • Phosphoprotein enriched in diabetes antibody
    see all

Anti-PEA15 (phospho S116) antibody images

  • ab59216 at 1/50 - 1/100 dilution staining PEA 15 in human skeletal muscle by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-PEA15 (phospho S116) antibody (ab59216)

ab59216 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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