• Product nameAnti-Pea3 antibody [1A2G3;4B11E5;7D2E5]
    See all Pea3 primary antibodies
  • Description
    Mouse monoclonal [1A2G3;4B11E5;7D2E5] to Pea3
  • Tested applicationsSuitable for: Flow Cyt, IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment: SEDLFQDLSH FQETWLAEAQ VPDSDEQFVP DFHSENLAFH SPTTRIKKEP QSPRTDPALS , corresponding to amino acids 50-109 of Human Pea3 expressed in E.Coli strain BL21 (DE3)

  • Positive control
    • Truncated Trx-Pea3 recombinant protein and full length Pea3 hIgGFc transfected CHO-K1 cell lysate.



Our Abpromise guarantee covers the use of ab70425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
ELISA 1/10000.


  • FunctionTranscriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
  • Sequence similaritiesBelongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenovirus E1A enhancer binding protein antibody
    • Adenovirus E1A enhancer-binding protein antibody
    • E1A F antibody
    • E1A-F antibody
    • ETS translocation variant 4 antibody
    • ets variant gene 4 (E1A enhancer binding protein E1AF) antibody
    • ETV4 antibody
    • ETV4_HUMAN antibody
    • PEA3 antibody
    • PEA3 protein antibody
    • PEAS3 antibody
    • Polyomavirus enhancer activator 3 antibody
    • Polyomavirus enhancer activator 3 homolog antibody
    • Protein PEA3 antibody
    see all

Anti-Pea3 antibody [1A2G3;4B11E5;7D2E5] images

  • All lanes : Anti-Pea3 antibody [1A2G3;4B11E5;7D2E5] (ab70425) at 1/500 dilution

    Lane 1 : Truncated Trx-Pea3 recombinant protein
    Lane 2 : Full length Pea3 hIgGFc transfected CHO-K1 cell lysate

    Predicted band size : 54 kDa
    Observed band size : 24,60 kDa (why is the actual band size different from the predicted?)
  • Anti-Pea3 antibody [1A2G3;4B11E5;7D2E5] (ab70425) at 1/2000 dilution + Cell lysates prepared from K562 cells at 100 µg

    HRP-conjugated Goat polyclonal to mouse IgG1

    Predicted band size : 54 kDa
  • ab70425 staining Pea3 in Human bladder transitional cell carcinoma tissue by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed with formalin overnight and subjected to heat mediated antigen retrieval in citrate buffer prior to blocking with 5% BSA for 30 minutes. The primary antibody was diluted 1/200 (5% BSA in PSA) and incubated with the sample for 1 hour at 20°C. An HRP-conjugated Goat anti-Mouse polyclonal was used undiluted as the secondary antibody.

    See Abreview

  • Overlay histogram showing K562 cells stained with ab70425 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab70425, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Pea3 antibody [1A2G3;4B11E5;7D2E5] (ab70425)

This product has been referenced in:
  • Gysin S  et al. Analysis of mRNA profiles after MEK1/2 inhibition in human pancreatic cancer cell lines reveals pathways involved in drug sensitivity. Mol Cancer Res 10:1607-19 (2012). WB ; Human . Read more (PubMed: 22833572) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (human bladder transitional cell carcinoma)
Specification human bladder transitional cell carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%

Dr. Imran Ahmad

Verified customer

Submitted Nov 29 2010