Overview

  • Product name
  • Description
    Rabbit polyclonal to Pea3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Pea3.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: MCF7; K562; Jurkat; HepG2.

Properties

Applications

Our Abpromise guarantee covers the use of ab98068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 54 kDa).

Target

  • Function
    Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
  • Sequence similarities
    Belongs to the ETS family.
    Contains 1 ETS DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Adenovirus E1A enhancer binding protein antibody
    • Adenovirus E1A enhancer-binding protein antibody
    • E1A F antibody
    • E1A-F antibody
    • ETS translocation variant 4 antibody
    • ets variant gene 4 (E1A enhancer binding protein E1AF) antibody
    • ETV4 antibody
    • ETV4_HUMAN antibody
    • PEA3 antibody
    • PEA3 protein antibody
    • PEAS3 antibody
    • Polyomavirus enhancer activator 3 antibody
    • Polyomavirus enhancer activator 3 homolog antibody
    • Protein PEA3 antibody
    see all

Images

  • All lanes : Anti-Pea3 antibody (ab98068) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 6 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 7 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml
    Lane 8 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Immunising peptide at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa,33 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References

ab98068 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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