The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 26 kDa. An additional band of unknown identity was also consistently observed at 30kDa. This band was successfully blocked by incubation with the immunizing peptide.
Use at an assay dependent concentration. PubMed: 21887552
RelevancePEBP4 appears to promote cellular resistance to TNF-induced apoptosis by inhibiting activation of the Raf-1/MEK/ERK pathway, JNK and phosphatidylethanolamine externalization.
ab99468 (5μg/ml) staining of PEBP4 in paraffin embedded Human Pancreas following microwaved antigen retrieval with Tris/EDTA
buffer pH9 and HRP-staining and shows staining consistant with lysosomes.
Western blot - PEBP4 antibody (ab99468)
Anti-PEBP4 antibody (ab99468) at 1 µg/ml + Human Heart lysates at 35 µg
Predicted band size : 26 kDa
References for Anti-PEBP4 antibody (ab99468)
This product has been referenced in:
Li H et al. Phosphatidylethanolamine-binding protein 4 is associated with breast cancer metastasis through Src-mediated Akt tyrosine phosphorylation. OncogeneN/A:N/A (2013).
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