Overview

  • Product name
  • Description
    Rabbit polyclonal to PECI
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 215-264 (AVGISVTLLG LFDAVYASDR ATFHTPFSHL GQSPEGCSSY TFPKIMSPAK) of Human PECI (NP_006108).

  • Positive control
    • HepG2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/62500.

Target

  • Function
    Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
  • Tissue specificity
    Abundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells.
  • Sequence similarities
    In the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
    Contains 1 ACB (acyl-CoA-binding) domain.
  • Cellular localization
    Mitochondrion and Peroxisome matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2-trans-enoyl-CoA isomerase antibody
    • ACBD2 antibody
    • acyl Coenzyme A binding domain containing 2 antibody
    • D2-enoyl-CoA isomerase antibody
    • D3 antibody
    • D3 D2 enoyl CoA isomerase antibody
    • DBI related protein 1 antibody
    • DBI-related protein 1 antibody
    • delta(2)-enoyl-CoA isomerase antibody
    • Delta(3) antibody
    • Delta(3) delta(2) enoyl CoA isomerase antibody
    • Diazepam-binding inhibitor-related protein 1 antibody
    • Dodecenoyl CoA isomerase antibody
    • Dodecenoyl-CoA isomerase antibody
    • DRS 1 antibody
    • DRS-1 antibody
    • DRS1 antibody
    • Eci2 antibody
    • ECI2_HUMAN antibody
    • Enoyl-CoA delta isomerase 2 antibody
    • HCA88 antibody
    • Hepatocellular carcinoma associated antigen 88 antibody
    • Hepatocellular carcinoma-associated antigen 88 antibody
    • mitochondrial antibody
    • pECI antibody
    • Peroxisomal 3 antibody
    • Peroxisomal 3,2-trans-enoyl-CoA isomerase antibody
    • Peroxisomal D3 D2 enoyl CoA isomerase antibody
    • Renal carcinoma antigen NY REN 1 antibody
    • Renal carcinoma antigen NY-REN-1 antibody
    see all

Images

  • Anti-PECI antibody (ab83029) at 1 µg/ml (5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)

References

ab83029 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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