• Product name
    Anti-PEDF antibody [1C4]
    See all PEDF primary antibodies
  • Description
    Mouse monoclonal [1C4] to PEDF
  • Tested applications
    Suitable for: ELISA, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 19-418 of Human PEDF purified from E. coli.

  • Positive control
    • A549 lysate; Human Kidney and Placenta tissues.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab157207 as a replacement.



Our Abpromise guarantee covers the use of ab115489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
Flow Cyt Use 0.1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/500 - 1/2000. Predicted molecular weight: 46 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity
    Retinal pigment epithelial cells and blood plasma.
  • Sequence similarities
    Belongs to the serpin family.
  • Developmental stage
    Expressed in quiescent cells.
  • Domain
    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • Post-translational
    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 35 protein antibody
    • EPC 1 antibody
    • EPC-1 antibody
    • EPC1 antibody
    • OI12 antibody
    • OI6 antibody
    • PEDF antibody
    • PEDF_HUMAN antibody
    • PIG 35 antibody
    • PIG35 antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • Proliferation inducing protein 35 antibody
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • Serpin F1 antibody
    • Serpin family F member 1 antibody
    • Serpin peptidase inhibitor clade F member 1 antibody
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • SERPINF 1 antibody
    • Serpinf1 antibody
    see all


  • Overlay histogram showing K562 cells stained with ab115489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab115489, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • Anti-PEDF antibody [1C4] (ab115489) at 1/1000 dilution + A549 cell lysate
    Developed using the ECL technique

    Predicted band size : 46 kDa
  • ab115489 10 ug/ml, staining PEDF in Formalin-fixed, Paraffin-embedded Human kidney tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • ab115489 10 ug/ml, staining PEDF in Formalin-fixed, Paraffin-embedded Human Placenta tissue by Immunohistochemistry, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.


This product has been referenced in:
  • Qian J  et al. Downregulation of HIF-1a inhibits the proliferation and invasion of non-small cell lung cancer NCI-H157 cells. Oncol Lett 11:1738-1744 (2016). Read more (PubMed: 26998070) »

See 1 Publication for this product

Customer reviews and Q&As

Gracias por contactarnos.

Efectivamente este anticuerpo ha sido validado en nuestro laboratorio en ensayos de ELISA indirecto. Cubriendo la placa con el antígeno, incubando con el primario, y detectando posteriormente con un secundario...

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I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please s...

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Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:
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