Overview

  • Product nameAnti-PEDF antibody
    See all PEDF primary antibodies
  • Description
    Rabbit polyclonal to PEDF
  • SpecificityRecognizes PEDF at 50kDa. Additional bands were detected at 66 and 76kDa, which were competed away by immunogen. These bands may be post translationally modified forms.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    His tagged fusion protein corresponding to full length human PEDF (Pigment Epithelial Derived Factor) expressed in baby hamster kidney cells.

  • Positive control
    • RIPA lysates from Y79 cells.
  • General notesFor maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Properties

Applications

Our Abpromise guarantee covers the use of ab14993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

Target

  • FunctionNeurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificityRetinal pigment epithelial cells and blood plasma.
  • Sequence similaritiesBelongs to the serpin family.
  • Developmental stageExpressed in quiescent cells.
  • DomainThe N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • Post-translational
    modifications
    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localizationSecreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 35 protein antibody
    • EPC 1 antibody
    • EPC-1 antibody
    • EPC1 antibody
    • OI12 antibody
    • OI6 antibody
    • PEDF antibody
    • PEDF_HUMAN antibody
    • PIG 35 antibody
    • PIG35 antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • Proliferation inducing protein 35 antibody
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • Serpin F1 antibody
    • Serpin family F member 1 antibody
    • Serpin peptidase inhibitor clade F member 1 antibody
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • SERPINF 1 antibody
    • Serpinf1 antibody
    see all

References for Anti-PEDF antibody (ab14993)

This product has been referenced in:
  • Tripathy D  et al. Age-related decrease in cerebrovascular-derived neuroprotective proteins: effect of acetaminophen. Microvasc Res 84:278-85 (2012). ELISA ; Rat . Read more (PubMed: 22944728) »
  • Simper D  et al. Comparative proteomics profiling reveals role of smooth muscle progenitors in extracellular matrix production. Arterioscler Thromb Vasc Biol 30:1325-32 (2010). WB ; Human . Read more (PubMed: 20431068) »

See all 2 Publications for this product

Product Wall




I am very pleased to hear you would like to accept our offer and test ab14993, ab30788 ,ab64053 and ab115489 in sELISA. These codes will give you: 1 freeprimary antibody per codebefore the expiration date. To redeem this offer, please s...

Read More

Thank you very much for your interest in PEDF and Somatostatin antibodies.

To our knowledge,none of our antibodies have been tested in sELISA. However, I have picked out four antibodies, which I found most suitable for this purpose:
Read More

Thank you for contacting us.

Here are the links to the databases that could help identify potential tissue or cell types that might not express the PEDF protein.

UniProt: http://www.uniprot.org/uniprot/P36955
Human Protein Atl...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (Cartilage)
Loading amount 25 µg
Specification Cartilage
Gel Running Conditions Reduced Denaturing (4-20% gradient gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. john wardale

Verified customer

Submitted Sep 24 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"