Overview

  • Product name
  • Description
    Rabbit polyclonal to PEDF
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Horse, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human PEDF.

    (Peptide available as ab74437.)

  • Positive control
    • This antibody gave a positive signal in Human Liver Tissue Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab45781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Target

  • Function
    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
  • Tissue specificity
    Retinal pigment epithelial cells and blood plasma.
  • Sequence similarities
    Belongs to the serpin family.
  • Developmental stage
    Expressed in quiescent cells.
  • Domain
    The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity.
  • Post-translational
    modifications
    The N-terminus is blocked. Extracellular phosphorylation enhances antiangiogenic activity.
    N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
  • Cellular localization
    Secreted. Melanosome. Enriched in stage I melanosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation-inducing gene 35 protein antibody
    • EPC 1 antibody
    • EPC-1 antibody
    • EPC1 antibody
    • OI12 antibody
    • OI6 antibody
    • PEDF antibody
    • PEDF_HUMAN antibody
    • PIG 35 antibody
    • PIG35 antibody
    • Pigment epithelium derived factor antibody
    • Pigment epithelium-derived factor antibody
    • Proliferation inducing protein 35 antibody
    • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • Serpin F1 antibody
    • Serpin family F member 1 antibody
    • Serpin peptidase inhibitor clade F member 1 antibody
    • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 antibody
    • SERPINF 1 antibody
    • Serpinf1 antibody
    see all

Images

  • Anti-PEDF antibody (ab45781) at 1 µg/ml + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 46 kDa
    Observed band size : 46 kDa


    Exposure time : 15 minutes

References

ab45781 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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