Recombinant
RabMAb

Anti-PEG10 antibody [EPR8156(2)] (ab181249)

Overview

  • Product name
    Anti-PEG10 antibody [EPR8156(2)]
    See all PEG10 primary antibodies
  • Description
    Rabbit monoclonal [EPR8156(2)] to PEG10
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PEG10 aa 100-200. The exact sequence is proprietary.
    Database link: Q86TG7

  • Positive control
    • WB: JAR, 293, HeLa and HepG2 cell lysates. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/80.
WB 1/1000 - 1/10000. Detects a band of approximately 50, 100 kDa (predicted molecular weight: 80 kDa).
ICC/IF 1/100 - 1/250.

Target

  • Function
    Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
  • Tissue specificity
    Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).
  • Sequence similarities
    Contains 1 CCHC-type zinc finger.
  • Developmental stage
    Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.
  • Post-translational
    modifications
    Isoform RF1/RF2 undergoes proteolytic cleavage.
  • Cellular localization
    Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA407948 antibody
    • Edr antibody
    • Embryonal carcinoma differentiation regulated antibody
    • Embryonal carcinoma differentiation-regulated protein antibody
    • HB 1 antibody
    • HB1 antibody
    • KIAA1051 antibody
    • Mammalian retrotransposon-derived protein 2 antibody
    • Mar2 antibody
    • Mart2 antibody
    • MEF3 like 1 antibody
    • MEF3-like protein 1 antibody
    • MEF3L antibody
    • MEF3L1 antibody
    • MyEF 3 antibody
    • Myelin expression factor 3-like protein 1 antibody
    • Paternally expressed 10 antibody
    • Paternally expressed gene 10 ORF1 antibody
    • Paternally expressed gene 10 protein antibody
    • Peg10 antibody
    • PEG10 protein antibody
    • PEG10_HUMAN antibody
    • Putative uncharacterized protein PEG10 antibody
    • Retrotransposon gag domain containing 3 antibody
    • Retrotransposon gag domain-containing protein 3 antibody
    • Retrotransposon-derived gag-like polyprotein antibody
    • Retrotransposon-derived protein PEG10 antibody
    • RGAG3 antibody
    • Ty3/Gypsy-like protein antibody
    see all

Images

  • Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PEG10 with ab181249 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling PEG10 (red) with ab181249 at a 1/80 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • All lanes : Anti-PEG10 antibody [EPR8156(2)] (ab181249) at 1/2000 dilution

    Lane 1 : JAR cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 80 kDa

References

ab181249 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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