Recombinant
RabMAb

Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833)

Overview

  • Product name
    Anti-Pellino 1 + Pellino 2 antibody [EPR19282]
  • Description
    Rabbit monoclonal [EPR19282] to Pellino 1 + Pellino 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Pellino 1 aa 150-250. The exact sequence is proprietary.
    Database link: Q96FA3

  • Positive control
    • WB: Human Pellino 1 and Pellino 2 full length recombinant proteins; Ramos, PC-12, Jurkat, Daudi, HeLa, C6 and NIH/3T3 whole cell lysates; RAW 264.7 treated with 1 µg/ml LPS for 6 hours whole cell lysate; Mouse and Rat brain lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab192833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).

Images

  • All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/5000 dilution

    Lane 1 : Human Pellino 1 full length recombinant protein
    Lane 2 : Human Pellino 2 full length recombinant protein
    Lane 3 : Human Pellino 3 full length recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa

    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1:1 second; Lane 2 and 3:1 minute.

    Human Pellino 1 full length recombinant protein contains aa1-418 with a His-Tag®. Human Pellino 2 full length recombinant protein contains aa1-420 with a His-Tag®. Human Pellino 3  full length recombinant protein contains aa1-469 with a His-Tag®. All three recombinant proteins were made in-house.

  • All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Untreated RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) treated with 1 µg/ml LPS for 6 hours, whole cell lysate
    Lane 4 : Untreated PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma cell line) treated with 1 µg/ml LPS for 6 hours whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa


    Exposure time : 3 minutes

    Blocking/dilution buffer: 5% NFDM/TBST.

    The expression of Pellino 1 can be induced by LPS. (PMID: 21204785).

  • All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Daudi (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa


    Exposure time : 3 minutes

    Blocking/dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Pellino 1 + Pellino 2 antibody [EPR19282] (ab192833) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Rat brain lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 46 kDa
    Observed band size : 46 kDa


    Exposure time : 3 minutes

    Blocking/dilution buffer: 5% NFDM/TBST.

References

ab192833 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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