1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionRequired for normal chromosome segregation during cell division and genomic stability (By similarity). May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the eukaryotic release factor 1 family. Pelota subfamily.
DomainThe N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity.