Overview

  • Product name
  • Description
    Rabbit polyclonal to PELO
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 356-385 of Human PELO

  • Positive control
    • HeLa cell lysate, Human Brain Tissue

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Required for normal chromosome segregation during cell division and genomic stability (By similarity). May function in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs. May have ribonuclease activity.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Belongs to the eukaryotic release factor 1 family. Pelota subfamily.
  • Domain
    The N-terminal domain has the RNA-binding Sm fold. It may harbor the endoribonuclease activity.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 17 antibody
    • CGI17 antibody
    • pelo antibody
    • PELO_HUMAN antibody
    • pelota (Drosophila) homolog antibody
    • pelota homolog antibody
    • PRO1770 antibody
    • Protein pelota homolog antibody
    see all

Images

  • Anti-PELO antibody (ab103061) at 1/100 dilution + HeLa cell lysate at 35 µg

    Predicted band size : 43 kDa
  • Formalin-fixed and paraffin-embedded Human brain tissue, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining, using ab103061 at a dilution of 1/50.

References

ab103061 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab103061.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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