Overview

  • Product name
  • Description
    Rabbit polyclonal to PELP1
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PELP1 aa 751-779 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8IZL8

  • Positive control
    • Human breast carcinoma tissue and mouse spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab170525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 120 kDa.

Target

  • Function
    Coactivator of estrogen receptor-mediated transcription and a corepressor of other nuclear hormone receptors and sequence-specific transcription factors. Plays a role in estrogen receptor (ER) genomic activity when present in the nuclear compartment by activating the ER target genes in a hormonal stimulation dependent manner. Can facilitate ER non-genomic signaling via SRC and PI3K interaction in the cytosol. Plays a role in E2-mediated cell cycle progression by interacting with RB1. May have important functional implications in ER/growth factor cross-talk. Interacts with several growth factor signaling components including EGFR and HRS. Involved in nuclear receptor signaling via its interaction with AR and NR3C1. May promote tumorigenesis via its interaction with and modulation of several oncogenes including SRC, PI3K, STAT3 and EGFR. Plays a role in cancer cell metastasis via its ability to modulate E2-mediated cytoskeleton changes and cell migration via its interaction with SRC and PI3K.
  • Tissue specificity
    Isoform 2 is expressed in breast cancer cell lines. Isoform 1 is widely expressed.
  • Domain
    The Glu-rich region mediates histones interaction.
    The Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are required for the association with nuclear receptor ESR1.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus. Cytoplasm. Also found associated with the plasma membrane. Mainly in cytoplasm in a subset of breast tumors. Localization is widely deregulated in endometrial cancers with predominantly cytoplasm localization in high-grade endometrial tumors.
  • Information by UniProt
  • Database links
  • Alternative names
    • glutamic acid- and leucine-rich protein 1 antibody
    • HMX3 antibody
    • MNAR antibody
    • Modulator of non-genomic activity of estrogen receptor antibody
    • Modulator of nongenomic activity of estrogen receptor antibody
    • P160 antibody
    • Pelp1 antibody
    • PELP1 proline glutamic acid leucine rich protein 1 antibody
    • PELP1_HUMAN antibody
    • proline and glutamic acid rich nuclear protein antibody
    • Proline glutamate and leucine rich protein 1 antibody
    • Proline- antibody
    • proline-, glutamic acid- and leucine-rich protein 1 antibody
    • Transcription factor HMX3 antibody
    see all

Images

  • Anti-PELP1 antibody (ab170525) at 1/100 dilution + Mouse spleen tissue lysate at 35 µg

    Predicted band size : 120 kDa
  • Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human breast carcinoma tissue, labeling PELP1 using ab170525 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170525 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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