Overview

  • Product name
  • Description
    Rabbit polyclonal to PEN2
  • Specificity
    ab18189 specifically detects mouse 12kDa Pen2 protein in lung lysate. A number of higher MW bands are also detected (~50 and ~100kDa bands) which may be because a proportion of Pen2 is found bound to components of the gamma secretase complex (see the Relevance section). All bands detected with ab18189 in mouse lung lysate were blocked with the immunising peptide ab19147. Human Pen2 protein was also detected (weakly) in lung lysates with ab18189 (2ug/ml) in our hands and all detected bands blocked by immunising peptide ab19147.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PEN2.

    (Peptide available as ab19147.)

  • Positive control
    • For endogenous sample testing in (e.g. in lung, spleen or brain tissue), it is suggested that the investigator load a greater amount of protein on the gel and combine immunoprecipitation with Western blot analysis. This antibody gave a positive result in HeLa cell line in ICC/IF
  • General notes


    PEN2 is a functional component of the gamma-secretase complex; coimmunoprecipitation of endogenous PEN2 and PS1 fragments using PS1 antibodies has been reported (Steiner et al, 2002).

Properties

Applications

Our Abpromise guarantee covers the use of ab18189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 11.9 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
  • Tissue specificity
    Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
  • Sequence similarities
    Belongs to the PEN-2 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma secretase subunit PEN 2 antibody
    • Gamma Secretase Subunit PEN2 antibody
    • Gamma-secretase subunit PEN-2 antibody
    • Hematopoietic stem/progenitor cells protein MDS033 antibody
    • MDS033 antibody
    • MSTP064 antibody
    • PEN 2 antibody
    • PEN2_HUMAN antibody
    • Presenilin Enhancer 2 antibody
    • Presenilin enhancer 2 homolog antibody
    • Presenilin enhancer protein 2 antibody
    • PSEN2 antibody
    • psenen antibody
    see all

Anti-PEN2 antibody images

  • All lanes : Anti-PEN2 antibody (ab18189) at 2 µg/ml

    Lane 1 : Mouse Lung Lysate
    Lane 2 : Mouse Lung Lysate with Human PEN2 peptide (ab19147) at 1 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Alexa Fluor Goat polyclonal to Rabbit at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
    Additional bands at : 50-60 kDa,95-100 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab18189 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18189, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-PEN2 antibody (ab18189)

This product has been referenced in:
  • Greenough MA  et al. Presenilins Promote the Cellular Uptake of Copper and Zinc and Maintain Copper Chaperone of SOD1-dependent Copper/Zinc Superoxide Dismutase Activity. J Biol Chem 286:9776-86 (2011). WB ; Human . Read more (PubMed: 21239495) »
  • Placanica L  et al. Characterization of an atypical gamma-secretase complex from hematopoietic origin. Biochemistry 49:2796-804 (2010). WB ; Human . Read more (PubMed: 20178366) »

See all 3 Publications for this product

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12%)
Loading amount
20 µg
Specification
MEF
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Nov 25 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 04 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Carcinoma)
Specification
Breast Carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Feb 25 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Brain membrane preparations)
Loading amount
20 µg
Specification
Brain membrane preparations
Gel Running Conditions
Reduced Denaturing (12% Tris-glycine)
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
Username

Mr. Michael Ho

Verified customer

Submitted Jul 10 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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