The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
Use a concentration of 20 µg/ml.
FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
Tissue specificityWidely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
Sequence similaritiesBelongs to the PEN-2 family.
Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
Hematopoietic stem/progenitor cells protein MDS033 antibody
PEN 2 antibody
Presenilin Enhancer 2 antibody
Presenilin enhancer 2 homolog antibody
Presenilin enhancer protein 2 antibody
Anti-PEN2 antibody - Carboxyterminal end images
Western blot - PEN2 antibody (ab62514)
Lane 1 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 0.5 µg/ml Lane 2 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 1 µg/ml Lane 3 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 2 µg/ml
Lane 1 : K562 cell lysate Lane 2 : K562 cell lysate Lane 3 : K562 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 12 kDa Observed band size : 12 kDa