Overview

  • Product nameAnti-PEN2 antibody - Carboxyterminal end
    See all PEN2 primary antibodies
  • Description
    Rabbit polyclonal to PEN2 - Carboxyterminal end
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) of 13 amino acids from near the C-terminus of PEN2

  • Positive control
    • K562 cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 20 µg/ml.

Target

  • FunctionEssential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
  • Tissue specificityWidely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
  • Sequence similaritiesBelongs to the PEN-2 family.
  • Cellular localizationEndoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • Gamma secretase subunit PEN 2 antibody
    • Gamma Secretase Subunit PEN2 antibody
    • Gamma-secretase subunit PEN-2 antibody
    • Hematopoietic stem/progenitor cells protein MDS033 antibody
    • MDS033 antibody
    • MSTP064 antibody
    • PEN 2 antibody
    • PEN2_HUMAN antibody
    • Presenilin Enhancer 2 antibody
    • Presenilin enhancer 2 homolog antibody
    • Presenilin enhancer protein 2 antibody
    • PSEN2 antibody
    • psenen antibody
    see all

Anti-PEN2 antibody - Carboxyterminal end images

  • Lane 1 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 0.5 µg/ml
    Lane 2 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 1 µg/ml
    Lane 3 : Anti-PEN2 antibody - Carboxyterminal end (ab62514) at 2 µg/ml

    Lane 1 : K562 cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 12 kDa
    Observed band size : 12 kDa
  • Immunofluorescence of K562 cells using ab62514 at 20 µg/ml.

References for Anti-PEN2 antibody - Carboxyterminal end (ab62514)

This product has been referenced in:
  • Area-Gomez E  et al. Presenilins are enriched in endoplasmic reticulum membranes associated with mitochondria. Am J Pathol 175:1810-6 (2009). WB ; Mouse . Read more (PubMed: 19834068) »

See 1 Publication for this product

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (MEF)
Gel Running Conditions Non-reduced Denaturing (12%)
Loading amount 20 µg
Specification MEF
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
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Submitted Nov 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"