Recombinant
RabMAb

Anti-PEN2 antibody [EPR9200] (ab154830)

Overview

  • Product name
    Anti-PEN2 antibody [EPR9200]
    See all PEN2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9200] to PEN2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human PEN2 (UniProt Q9NZ42).

  • Positive control
    • Neuro-2a, fetal lung and Human placenta lysates; Human skeletal muscle tissue; Neuro-2a cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab154830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase activity.
    • Tissue specificity
      Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen thymus, skeletal muscle, heart and brain.
    • Sequence similarities
      Belongs to the PEN-2 family.
    • Cellular localization
      Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly located in the endoplasmic reticulum and in the cis-Golgi.
    • Information by UniProt
    • Database links
    • Alternative names
      • Gamma secretase subunit PEN 2 antibody
      • Gamma Secretase Subunit PEN2 antibody
      • Gamma-secretase subunit PEN-2 antibody
      • Hematopoietic stem/progenitor cells protein MDS033 antibody
      • MDS033 antibody
      • MSTP064 antibody
      • PEN 2 antibody
      • PEN2_HUMAN antibody
      • Presenilin Enhancer 2 antibody
      • Presenilin enhancer 2 homolog antibody
      • Presenilin enhancer protein 2 antibody
      • PSEN2 antibody
      • psenen antibody
      see all

    Images

    • All lanes : Anti-PEN2 antibody [EPR9200] (ab154830) at 1/1000 dilution

      Lane 1 : Neuro-2a cell lysate
      Lane 2 : Fetal lung lysate
      Lane 3 : Human placenta lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 12 kDa
    • Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling PEN2 with ab154830 at 1/100 dilution.
    • Flow cytometric analysis of permeabilized Neuro-2a cells labeling PEN2 with ab154830 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:
    • Xu TH  et al. Alzheimer's disease-associated mutations increase amyloid precursor protein resistance to ?-secretase cleavage and the Aß42/Aß40 ratio. Cell Discov 2:16026 (2016). WB ; Human . Read more (PubMed: 27625790) »
    • Zhang X  et al. The topology of pen-2, a ?-secretase subunit, revisited: evidence for a reentrant loop and a single pass transmembrane domain. Mol Neurodegener 10:39 (2015). Read more (PubMed: 26296997) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 04 2013

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Oct 04 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Sample
    Human Tissue sections (Placenta)
    Specification
    Placenta
    Permeabilization
    No
    Fixative
    Formaldehyde
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Jun 20 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up