Recombinant
RabMAb

Anti-Pentraxin 3 antibody [EPR18678-105] (ab190838)

Overview

  • Product name
    Anti-Pentraxin 3 antibody [EPR18678-105]
    See all Pentraxin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR18678-105] to Pentraxin 3
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, WB, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human Pentraxin 3 aa 1-200. The exact sequence is proprietary.
    Database link: P26022

  • Positive control
    • WB: HUVEC whole cell lysate (+/- treatment with LPS and BFA). ICC/IF: HUVEC cells. Flow cytometry: HUVEC cells. IP: HUVEC whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab190838 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/60.
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IP 1/30.

Target

  • Function
    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
  • Sequence similarities
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI607804 antibody
    • Pentaxin 3 antibody
    • Pentaxin-related protein PTX3 antibody
    • Pentraxin 3 long antibody
    • Pentraxin-related gene antibody
    • Pentraxin-related gene rapidly induced by IL-1 beta antibody
    • Pentraxin-related protein PTX3 antibody
    • PTX3 antibody
    • PTX3_HUMAN antibody
    • TNF alpha-induced protein 5 antibody
    • TNFAIP5 antibody
    • TSG-14 antibody
    • TSG14 antibody
    • Tumor necrosis factor alpha-induced protein 5 antibody
    • Tumor necrosis factor-inducible gene 14 protein antibody
    • Tumor necrosis factor-inducible protein TSG-14 antibody
    see all

Images

  • All lanes : Anti-Pentraxin 3 antibody [EPR18678-105] (ab190838) at 1/1000 dilution

    Lane 1 : Untreated HUVEC (human umbilical vein endothelial cell line) whole cell lysate
    Lane 2 : HUVEC treated with 0.5 ug/ml LPS for 4 hours followed by 300 ng/ml BFA for 20 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second

    Blocking and dilution buffer: 5% NFDM/TBST 

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3 with ab190838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HUVEC cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) secondary antibody at 1/200 dilution (red).


    -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

     

  • Anti-Pentraxin 3 antibody [EPR18678-105] (ab190838) at 1/200 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 41 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    Blocking and dilution buffer: 5% NFDM/TBST

    Higher concentration of this antibody is required for the detection of Pentraxin 3 in untreated cells in WB.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HUVEC (human umbilical vein endothelial cell line) cells labeling Pentraxin 3 with ab190838 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

  • Pentraxin 3 was immunoprecipitated from 0.35 mg of HUVEC (human umbilical vein endothelial cell line) whole cell lysate with ab190838 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab190838 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/1000 dilution.


    Lane 1: HUVEC whole cell lysate 10 μg (Input).
    Lane 2: ab190838 IP in HUVEC whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab190838 in HUVEC whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 17389238).

References

ab190838 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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