Anti-Pentraxin 3 antibody [EPR6699] (ab125007)


  • Product nameAnti-Pentraxin 3 antibody [EPR6699]
    See all Pentraxin 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR6699] to Pentraxin 3
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HL-60 treated with Dexamethasone, HL-60, Human spleen, NIH/3T3, and HUVEC lysates; HUVEC cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab125007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC or IHC-P.
  • Target

    • FunctionPlays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
    • Sequence similaritiesContains 1 pentaxin domain.
    • Post-translational
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • AI607804 antibody
      • Pentaxin 3 antibody
      • Pentaxin-related protein PTX3 antibody
      • Pentraxin 3 long antibody
      • Pentraxin-related gene antibody
      • Pentraxin-related gene rapidly induced by IL-1 beta antibody
      • Pentraxin-related protein PTX3 antibody
      • PTX3 antibody
      • PTX3_HUMAN antibody
      • TNF alpha-induced protein 5 antibody
      • TNFAIP5 antibody
      • TSG-14 antibody
      • TSG14 antibody
      • Tumor necrosis factor alpha-induced protein 5 antibody
      • Tumor necrosis factor-inducible gene 14 protein antibody
      • Tumor necrosis factor-inducible protein TSG-14 antibody
      see all

    Anti-Pentraxin 3 antibody [EPR6699] images

    • Overlay histogram showing HUVEC cells stained with ab125007 (red line) at 1/40 dilution. The cells were fixed with 2% paraformaldehyde. The secondary antibody used was a FITC conjugated goat anti-rabbit IgG at 1/150 dilution. Isotype control antibody (black line) was rabbit monoclonal IgG used under the same conditions.

    • Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/10000 dilution + NIH/3T3 cell lysate at 20 µg

      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size : 42 kDa
    • Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/10000 dilution + HUVEC cell lysate at 20 µg

      Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size : 42 kDa
    • All lanes : Anti-Pentraxin 3 antibody [EPR6699] (ab125007) at 1/1000 dilution (unpurified)

      Lane 1 : HL-60 treated with Dexamethasone lysate
      Lane 2 : HL-60 lysate
      Lane 3 : Human spleen lysate
      Lane 4 : NIH/3T3 cell lysate
      Lane 5 : HUVEC lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 42 kDa
      Observed band size : 42 kDa
    • Flow cytometric analysis of permeabilized HUVEC cells using ab125007, unpurified, at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Pentraxin 3 antibody [EPR6699] (ab125007)

    ab125007 has not yet been referenced specifically in any publications.

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (cardiac tissue)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 30 µg
    Treatment LPS treated mice
    Specification cardiac tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

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    Submitted May 07 2015