Overview

  • Product name
    Anti-Pentraxin 3 antibody [MNB1]
    See all Pentraxin 3 primary antibodies
  • Description
    Rat monoclonal [MNB1] to Pentraxin 3
  • Tested applications
    Suitable for: WB, IP, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant Human Pentraxin 3.

  • Positive control
    • ICC/IF: MDA-MB-231 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab90806 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Predicted molecular weight: 42 kDa.
IP Use at an assay dependent dilution.
IHC-Fr 1/50.
IHC-P 1/50.

Target

  • Function
    Plays a role in the regulation of innate resistance to pathogens, inflammatory reactions, possibly clearance of self-components and female fertility.
  • Sequence similarities
    Contains 1 pentaxin domain.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI607804 antibody
    • Pentaxin 3 antibody
    • Pentaxin-related protein PTX3 antibody
    • Pentraxin 3 long antibody
    • Pentraxin-related gene antibody
    • Pentraxin-related gene rapidly induced by IL-1 beta antibody
    • Pentraxin-related protein PTX3 antibody
    • PTX3 antibody
    • PTX3_HUMAN antibody
    • TNF alpha-induced protein 5 antibody
    • TNFAIP5 antibody
    • TSG-14 antibody
    • TSG14 antibody
    • Tumor necrosis factor alpha-induced protein 5 antibody
    • Tumor necrosis factor-inducible gene 14 protein antibody
    • Tumor necrosis factor-inducible protein TSG-14 antibody
    see all

Images

  • ICC/IF image of ab90806 stained MDA-MB-231 cells. The cells were 4% parafrmaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90806, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab98420, DyLight® 488 goat anti-rat IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Mayer C  et al. Sterile inflammation as a factor in human male infertility: Involvement of Toll like receptor 2, biglycan and peritubular cells. Sci Rep 6:37128 (2016). WB ; Human . Read more (PubMed: 27849015) »
  • Belone Ade F  et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . Read more (PubMed: 26635870) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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