Overview

  • Product name
    Anti-PEP1 antibody [18C8BC7AD10]
  • Description
    Mouse monoclonal [18C8BC7AD10] to PEP1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein

  • Positive control
    • Yeast lysate
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    Near homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
  • Clonality
    Monoclonal
  • Clone number
    18C8BC7AD10
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).

Target

  • Function
    Functions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.
  • Sequence similarities
    Belongs to the VPS10-related sortilin family.
    Contains 10 BNR repeats.
  • Domain
    The lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.
  • Cellular localization
    Golgi apparatus > trans-Golgi network membrane. Prevacuolar compartment membrane. Cycles between the Golgi apparatus and the prevacuolar compartments.
  • Information by UniProt
  • Database links
    • Alternative names
      • Carboxypeptidase Y receptor antibody
      • Carboxypeptidase Y-deficient protein 1 antibody
      • CPY receptor antibody
      • pep1 antibody
      • Sortilin VPS10 antibody
      • Vacuolar carboxypeptidase sorting receptor VPS10 antibody
      • Vacuolar protein sorting-associated protein 10 antibody
      • Vacuolar protein sorting/targeting protein VPS10 antibody
      • Vacuolar protein-targeting protein 1 antibody
      • VPS10_YEAST antibody
      see all

    Images

    • Anti-PEP1 antibody [18C8BC7AD10] (ab113690) + Yeast whole cell lysate at 15 µg
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 177 kDa

    References

    This product has been referenced in:
    • Aufschnaiter A  et al. The Coordinated Action of Calcineurin and Cathepsin D Protects Against a-Synuclein Toxicity. Front Mol Neurosci 10:207 (2017). Read more (PubMed: 28713240) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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