Anti-PEP1 antibody [18C8BC7AD10] (ab113690)

Overview

  • Product nameAnti-PEP1 antibody [18C8BC7AD10]
  • Description
    Mouse monoclonal [18C8BC7AD10] to PEP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein

  • Positive control
    • Yeast lysate
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituent: HBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesNear homogeneity. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation.
  • ClonalityMonoclonal
  • Clone number18C8BC7AD10
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 4 µg/ml. Detects a band of approximately 177 kDa (predicted molecular weight: 177 kDa).

Target

  • FunctionFunctions as a sorting receptor in the Golgi compartment required for the intracellular sorting and delivery of soluble vacuolar proteins, like carboxypeptidase Y (CPY) and proteinase A. May execute multiple rounds of sorting by cycling between the late Golgi and a prevacuolar endosome-like compartment. Binds the Golgi-modified P2 form of CPY, and this interaction is dependent on the presence of an intact CPY vacuolar protein sorting signal.
  • Sequence similaritiesBelongs to the VPS10-related sortilin family.
    Contains 10 BNR repeats.
  • DomainThe lumenal domain contains two regions of approximately 650 AA that exhibit 20% identity. The cytoplasmic domain serves as a Golgi retention/recycling signal.
  • Cellular localizationGolgi apparatus > trans-Golgi network membrane. Prevacuolar compartment membrane. Cycles between the Golgi apparatus and the prevacuolar compartments.
  • Information by UniProt
  • Database links
    • Alternative names
      • Carboxypeptidase Y receptor antibody
      • Carboxypeptidase Y-deficient protein 1 antibody
      • CPY receptor antibody
      • pep1 antibody
      • Sortilin VPS10 antibody
      • Vacuolar carboxypeptidase sorting receptor VPS10 antibody
      • Vacuolar protein sorting-associated protein 10 antibody
      • Vacuolar protein sorting/targeting protein VPS10 antibody
      • Vacuolar protein-targeting protein 1 antibody
      • VPS10_YEAST antibody
      see all

    Anti-PEP1 antibody [18C8BC7AD10] images

    • Anti-PEP1 antibody [18C8BC7AD10] (ab113690) + Yeast whole cell lysate at 15 µg
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 177 kDa

    References for Anti-PEP1 antibody [18C8BC7AD10] (ab113690)

    ab113690 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"