Overview

  • Product name
  • Description
    Rabbit polyclonal to PEPD
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 22-290 of Human PEPD (BC028295).

  • Positive control
    • Human fetal liver tissue, HeLa and HepG2 cell lysates. IF/ICC: HepG2 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab111851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
  • Involvement in disease
    Defects in PEPD are a cause of prolidase deficiency (PD) [MIM:170100]. Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait.
  • Sequence similarities
    Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aminoacyl L proline hydrolase antibody
    • Imidodipeptidase antibody
    • MGC10905 antibody
    • MGC95081 antibody
    • Pep 4 antibody
    • Pep4 antibody
    • pepD antibody
    • PEPD_HUMAN antibody
    • Peptidase 4 antibody
    • Peptidase D antibody
    • Prolidase antibody
    • Proline dipeptidase antibody
    • X pro dipeptidase antibody
    • X-Pro dipeptidase antibody
    • Xaa-Pro dipeptidase antibody
    see all

Images

  • ab111851 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111851 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-PEPD antibody (ab111851) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate


    Predicted band size : 55 kDa
  • ab111851, at a 1/100 dilution, staining PEPD in Formalin-fixed, Paraffin-embedded Human fetal liver tissue by Immunohistochemistry.

References

ab111851 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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