Overview

  • Product name
  • Description
    Rabbit polyclonal to PEPD
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (GAVFMPHGLG HFLGIDVHDV GGYPEGVERI DEPGLRSLRT ARHLQPGMVL) of human PEPD (NP_000276).

  • Positive control
    • Human placenta lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 55 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.
  • Involvement in disease
    Defects in PEPD are a cause of prolidase deficiency (PD) [MIM:170100]. Prolidase deficiency is an autosomal recessive disorder associated with iminodipeptiduria. The clinical phenotype includes skin ulcers, mental retardation, recurrent infections, and a characteristic facies. These features, however are incompletely penetrant and highly variable in both age of onset and severity. There is a tight linkage between the polymorphisms of prolidase and the myotonic dystrophy trait.
  • Sequence similarities
    Belongs to the peptidase M24B family. Eukaryotic-type prolidase subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Aminoacyl L proline hydrolase antibody
    • Imidodipeptidase antibody
    • MGC10905 antibody
    • MGC95081 antibody
    • Pep 4 antibody
    • Pep4 antibody
    • pepD antibody
    • PEPD_HUMAN antibody
    • Peptidase 4 antibody
    • Peptidase D antibody
    • Prolidase antibody
    • Proline dipeptidase antibody
    • X pro dipeptidase antibody
    • X-Pro dipeptidase antibody
    • Xaa-Pro dipeptidase antibody
    see all

Images

  • Anti-PEPD antibody (ab86507) at 1 µg/ml + human placenta lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

References

This product has been referenced in:
  • Yang L  et al. A plasma proteolysis pathway comprising blood coagulation proteases. Oncotarget 7:40919-40938 (2016). Read more (PubMed: 27248165) »
  • Yang L  et al. Inhibition of ERBB2-overexpressing Tumors by Recombinant Human Prolidase and Its Enzymatically Inactive Mutant. EBioMedicine 2:396-405 (2015). Read more (PubMed: 26086037) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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