Anti-Pepsin antibody (HRP) (ab34531)

Overview

  • Product name
    Anti-Pepsin antibody (HRP)
    See all Pepsin primary antibodies
  • Description
    Goat polyclonal to Pepsin (HRP)
  • Conjugation
    HRP
  • Tested applications
    Suitable for: WB, ELISA, Dot blot, Electron Microscopymore details
  • Species reactivity
    Reacts with: Goat, Pig
  • Immunogen

    Pepsin from Porcine Stomach

Properties

Applications

Our Abpromise guarantee covers the use of ab34531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
Dot blot
Electron Microscopy
  • Application notes
    Dot: Use at an assay dependent dilution.
    Immunoperoxidase Electron Microscopy: Use at an assay dependent dilution.
    ELISA: 1/10000 - 1/50000.
    This product has been assayed against 1.0µg of Porcine Pepsin in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic-acid]) as a substrate for 30 minutes at RT.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 42 kDa.
    Suitable for other peroxidase-antibody based enzymatic assays.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Pepsin is a digestive enzyme with particularly broad specificity. It is stored as pepsinogen, so only released when needed. In the stomach, chief cells release pepsinogen. The hormone gastrin and the vagus nerve trigger the release of both pepsinogen and HCl from the stomach lining when food is ingested. HCl creates an acidic environment which allows pepsinogen to unfold and cleave itself in an autocatalytic fashion, thereby generating pepsin (the active form). Pepsin may then cleave the dietary proteins into smaller peptides.
    • Cellular localization
      Secreted
    • Database links
    • Alternative names
      • Pepsin A 3 antibody
      • Pepsin A 4 antibody
      • Pepsin A 5 antibody
      • Pepsin A antibody
      • Pepsin antibody
      • Pepsinogen 3 antibody
      • Pepsinogen 4 antibody
      • Pepsinogen 5 antibody
      • Pepsinogen antibody
      • PGA antibody
      • PGA3 antibody
      • PGA4 antibody
      • PGA5 antibody
      see all

    References

    ab34531 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Vielen Dank für Ihre Anfrage.
    Leider kann ich Ihnen nicht garantieren, dass diese Produkte für immer in unserem Katalog bleiben. Diese Entscheidung hängt von vielen Faktoren ab und wird von verschiedenen Abteilungen wie Marketing, Produktion etc. ...

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    Thank you for your inquiry.
    I am happy to confirm that ab34531 (Anti-Pepsin antibody (HRP)) is the HRP conjugated version of ab34566.
    Please follow this link link to the datasheet of ab34531:
    http://www.abcam.com/index.html?datasheet=345...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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