Anti-Pepsinogen I antibody [2F5] (ab50607)

Overview

  • Product nameAnti-Pepsinogen I antibody [2F5]
    See all Pepsinogen I primary antibodies
  • Description
    Mouse monoclonal [2F5] to Pepsinogen I
  • Tested applicationsSuitable for: Sandwich ELISA, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native Human Pepsinogen I protein (purified)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.15mM PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number2F5
  • IsotypeIgG2a
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab50607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
ELISA
  • Application notesELISA: Use at a concentration of 1 - 10 µg/ml.
    sELISA: Can be paired for ELISA with Mouse monoclonal [7G3] to Pepsinogen I (ab50123). ab50607 can be used as a capture antibody in a human Pepsinogen I ELISA in combination with monoclonal antibody ab50123.Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionShows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
    • Sequence similaritiesBelongs to the peptidase A1 family.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • PEPA3_HUMAN antibody
      • Pepsin A-3 antibody
      • Pepsinogen 3 group I antibody
      • PEPSINOGEN I--SECOND LOCUS antibody
      • Pepsinogen-3 antibody
      • PGA3 antibody
      see all

    References for Anti-Pepsinogen I antibody [2F5] (ab50607)

    ab50607 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call yesterday and for your patience while I have been in touch with the lab.

    The antibody was raised against full-length pepsinogen purified from human stomach, and it has been tested to detect pepsinogen from serum and ur...

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    Thank you for your patience. Regarding reactivity of this antibody, ab50607, with pepsin, the developer reports: We have never tested clone 2F5 against mature pepsin, so it is unknown if the epitope is on pepsin or not. We test anti pepsingen antibodie...

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    Thank you for your enquiry. I am sorry it has taken some time to confirm this information. I can confirm that for sandwich ELISA the following have been used for testing: Capture antibody : ab50607 Anti-Pepsinogen I antibody 2F5 Detection antib...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"