Anti-Pepsinogen I antibody [7G3] (ab50123)

Overview

  • Product nameAnti-Pepsinogen I antibody [7G3]
    See all Pepsinogen I primary antibodies
  • Description
    Mouse monoclonal [7G3] to Pepsinogen I
  • Tested applicationsSuitable for: IHC-P, ELISA, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified)

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.15M Sodium chloride, 15mM PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number7G3
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 1 - 10 µg/ml. Can be paired for ELISA with Mouse monoclonal [2F5] to Pepsinogen I (ab50607).
Sandwich ELISA Use at an assay dependent concentration.

Target

  • FunctionShows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
  • Sequence similaritiesBelongs to the peptidase A1 family.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • PEPA3_HUMAN antibody
    • Pepsin A-3 antibody
    • Pepsinogen 3 group I antibody
    • PEPSINOGEN I--SECOND LOCUS antibody
    • Pepsinogen-3 antibody
    • PGA3 antibody
    see all

Anti-Pepsinogen I antibody [7G3] images

  • IHC image of ab50123 staining in human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab50123, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Pepsinogen I antibody [7G3] (ab50123)

ab50123 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Intestine)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Specification Intestine
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative Paraformaldehyde
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Verified customer

Submitted Aug 03 2015

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
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Verified customer

Submitted Aug 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

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Verified customer

Submitted Apr 12 2012

Thank you for your enquiry. I am sorry it has taken some time to confirm this information. I can confirm that for sandwich ELISA the following have been used for testing: Capture antibody : ab50607 Anti-Pepsinogen I antibody 2F5 Detection antib...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"