Anti-Peptide YY/PYY antibody (ab22663)
Key features and details
- Rabbit polyclonal to Peptide YY/PYY
- Suitable for: IHC-FoFr, IHC-P
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-Peptide YY/PYY antibody
See all Peptide YY/PYY primary antibodies -
Description
Rabbit polyclonal to Peptide YY/PYY -
Host species
Rabbit -
Specificity
Immunostaining may be abolished by preincubation with 1nmol synthetic porcine PYY per mL diluted antibody. NPY and PP (10nmol/mL) have no effect on immunostaining. -
Tested applications
Suitable for: IHC-FoFr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Dog, Human -
Immunogen
Full length native protein (purified) corresponding to Pig Peptide YY/PYY aa 1-36.
Sequence:YPAKPEAPGE DASPEELSRY YASLRHYLNL VTRQRY
Database link: P68005 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.07% Sodium azide -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
Use at an assay dependent concentration.
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IHC-P | (4) |
Use at an assay dependent concentration.
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Notes |
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IHC-FoFr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility. -
Sequence similarities
Belongs to the NPY family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 607156 Dog
- Entrez Gene: 5697 Human
- Entrez Gene: 217212 Mouse
- Entrez Gene: 287730 Rat
- Omim: 600781 Human
- SwissProt: P68004 Dog
- SwissProt: P10082 Human
- SwissProt: Q9EPS2 Mouse
see all -
Alternative names
- GHYY antibody
- MGC19143 antibody
- Peptide tyrosine tyrosine antibody
see all
Images
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IHC image of Peptide YY/PYY staining in mouse colon formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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IHC image of Peptide YY/PYY staining in rat pancreas formalin fixed paraffin embedded tissue section. Antigen retrieval was heat mediated with citrate and sections were blocked in 1% B.S.A for 10 minutes at 21ºC. Sample was incubated in primary antibody at 1/9000 for 2 hours and a biotin conjugated goat-anti-rabbit secondary was used at 1/250.
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Adjacent de-waxed sections of Bouin-fixed fetal (14 day) rat pancreas stained with rabbit polyclonal antisera to PYY (ab22663; panel a) and glucagon (ab18461; panel b). Arrows indicate identical cell profiles. From Ali-Rachedi et al, 1984.
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ab22663 staining Peptide YY/PYY (green) in murine pancreas tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue (left, WT; right, Peptide YY/PYY KO) was fixed with paraformaldehyde and blocked with 3% H2O2 in methanol, followed by antigen retrieval with trypsin. Samples were incubated with primary antibody (1/2000 in TNT) overnight. An AlexaFluor®488-conjugated donkey anti-rabbit IgG (1/1000 in TNT) was used as the secondary antibody. Nuclei were stained with DAPI (red).
Datasheets and documents
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Datasheet download
References (19)
ab22663 has been referenced in 19 publications.
- Kim KS et al. Vertical sleeve gastrectomy induces enteroendocrine cell differentiation of intestinal stem cells through bile acid signaling. JCI Insight 7:N/A (2022). PubMed: 35503251
- Chu L et al. In vivo drug discovery for increasing incretin-expressing cells identifies DYRK inhibitors that reinforce the enteroendocrine system. Cell Chem Biol 29:1368-1380.e5 (2022). PubMed: 35998625
- Sridhar A et al. Differential effects of RYGB surgery and best medical treatment for obesity-diabetes on intestinal and islet adaptations in obese-diabetic ZDSD rats. PLoS One 17:e0274788 (2022). PubMed: 36137097
- Ichijo R et al. Vasculature-driven stem cell population coordinates tissue scaling in dynamic organs. Sci Adv 7:N/A (2021). PubMed: 33568475
- Camacho-Ramírez A et al. The Leading Role of Peptide Tyrosine Tyrosine in Glycemic Control After Roux-en-Y Gastric Bypass in Rats. Obes Surg 30:697-706 (2020). PubMed: 31701411