Overview

  • Product nameAnti-PER1 antibody
    See all PER1 primary antibodies
  • Description
    Rabbit polyclonal to PER1
  • SpecificityDetects PER 1 from mouse tissues as well as overexpressed recombinant mouse PER1.
  • Tested applicationsSuitable for: ICC, IP, ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Hamster, Human, Drosophila melanogaster
  • Immunogen

    Synthetic peptide corresponding to Mouse PER1 aa 39-51.
    Sequence:

    PSLADDTDANSNG


    (Peptide available as ab5856)

  • Positive control
    • mouse testes tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab3443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/10 - 1/200.
IP Use at an assay dependent concentration.
ICC/IF 1/10 - 1/200.
IHC-Fr 1/50 - 1/500.
WB 1/100 - 1/1000. Predicted molecular weight: 136 kDa.Can be blocked with PER1 peptide (ab5856).
IHC-P 1/50 - 1/500.

Target

  • FunctionComponent of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK
    NPAS2-BMAL1
    BMAL2-induced transactivation.
  • Tissue specificityWidely expressed. Found in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, kidney, spleen, thymus, prostate, testis, ovary and small intestine. Highest level in skeletal muscle. Low level in kidney.
  • Sequence similaritiesContains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • Post-translational
    modifications
    Phosphorylated on serine residues by CSNK1E. Also can be phosphorylated by the delta isoform. Phosphorylation by CSNK1 retains PER1 in the cytoplasm and leads to its ubiquitination and subsequent degradation. Phosphorylated upon DNA damage, probably by ATM or ATR.
    Ubiquitinated.
  • Cellular localizationNucleus. Cytoplasm. Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER1 nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2.
  • Information by UniProt
  • Database links
  • Alternative names
    • Circadian clock protein PERIOD 1 antibody
    • Circadian clock protein PERIOD1 antibody
    • Circadian pacemaker protein Rigui antibody
    • hPER 1 antibody
    • hPER antibody
    • hPER1 antibody
    • KIAA0482 antibody
    • MGC88021 antibody
    • PER 1 antibody
    • PER antibody
    • PER1 antibody
    • PER1 protein antibody
    • PER1_HUMAN antibody
    • Period 1 antibody
    • Period circadian protein homolog 1 antibody
    • Period drosophila homolog of antibody
    • Period homolog 1 antibody
    • Period1 antibody
    • RIGUI antibody
    see all

Anti-PER1 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of PER1 (green) showing staining in the cytoplasm and nucleus of SH-SY5Y cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with ab3443 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemsitry/Immunofluorescence analysis of PER1 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubatedwith ab3443 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunocytochemsitry/Immunofluorescence analysis of PER1 (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with an3443 in 3% BSA-PBS at a dilution of 1:100 overnight at 4 ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • ab3443 labelling PER1 in the nucleus of Mouse skeletal muscle tissue (right) compared with a negative control (left) by Immunohistchemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3443 labelling PER1 in the nucleus of Human skeletal muscle tissue (right) compared with a negative control (left) by Immunohistchemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3443 labelling PER1 in the nucleus of Human liver tissue (right) compared with a negative control (left) by Immunohistchemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit IgG was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3443 (4µg/ml) staining PER1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasm and nuclei in cells of the bronchioles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Anti-PER1 antibody (ab3443) at 1/500 dilution + Mouse testis cell lysate at 25 µg

    Predicted band size : 136 kDa
    Observed band size : 136 kDa

References for Anti-PER1 antibody (ab3443)

This product has been referenced in:
  • Zhao H  et al. Prognostic relevance of Period1 (Per1) and Period2 (Per2) expression in human gastric cancer. Int J Clin Exp Pathol 7:619-30 (2014). IHC-P ; Human . Read more (PubMed: 24551282) »
  • Hu ML  et al. Deregulated expression of circadian clock genes in gastric cancer. BMC Gastroenterol 14:67 (2014). IHC-Fr ; Human . Read more (PubMed: 24708606) »

See all 8 Publications for this product

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Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (hepatocytes)
Specification hepatocytes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Fixative Paraformaldehyde
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Submitted Dec 11 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 17 2014

Thank you for contacting Abcam regarding ab3443.


I am sorry that you have been experiencing difficulties with this antibody in WB. I have reviewed the protocol information you provided and I wanted to make some suggestions to improve yo...

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Thank you for your enquiry. We're currently unaware of any publications which feature the use of this antibody, and we don't have any images available at this time. Please contact us again if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"