Anti-PER3 antibody (ab224594)
Key features and details
- Rabbit polyclonal to PER3
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-PER3 antibody
See all PER3 primary antibodies -
Description
Rabbit polyclonal to PER3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human PER3 aa 1-150.
Database link: P56645 -
Positive control
- WB: HeLa whole cell lysate (ab150035); mouse skeletal muscle tissue lysate. IHC: Human ovarian cancer and colon cancer tissue lysate. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/200.
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
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Notes |
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IHC-P
1/20 - 1/200. |
ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa). |
Target
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Function
Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme. -
Sequence similarities
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
Post-translational
modificationsPhosphorylation appears to require association with PER1 and translocation to the nucleus.
Ubiquitinated. -
Cellular localization
Cytoplasm. Nucleus. Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS. - Information by UniProt
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Database links
- Entrez Gene: 8863 Human
- Entrez Gene: 18628 Mouse
- Omim: 603427 Human
- SwissProt: P56645 Human
- SwissProt: O70361 Mouse
- Unigene: 162200 Human
- Unigene: 121361 Mouse
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Alternative names
- 2810049O06Rik antibody
- Cell growth inhibiting gene 13 protein antibody
- Cell growth-inhibiting gene 13 protein antibody
see all
Images
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All lanes : Anti-PER3 antibody (ab224594) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : Mouse skeletal tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 132 kDa
Observed band size: 132 kDa -
Paraffin-embedded human ovarian cancer tissue stained for PER3 using ab224594 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon cancer tissue stained for PER3 using ab224594 at 1/100 dilution in immunohistochemical analysis.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for PER3 (green) using ab224594 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab224594 has been referenced in 2 publications.
- Wu X et al. A transcriptomics-guided drug target discovery strategy identifies receptor ligands for lung regeneration. Sci Adv 8:eabj9949 (2022). PubMed: 35319981
- Mosna K et al. Complex changes of circadian proteins expression in inflammatory bowel disease. Bratisl Lek Listy 122:235-241 (2021). PubMed: 33729814