Overview

  • Product namePerforin (PRF1) Human ELISA Kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    A 8 416pg/ml 20 5%
    B 8 674pg/ml 25 4%
    C 8 984pg/ml 24 3%
    A 8 416pg/ml 20 5%
    B 8 674pg/ml 25 4%
    C 8 984pg/ml 24 3%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 40 pg/ml
  • Range
    62.5 pg/ml - 2000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 71 % - %

  • Assay time
    2h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Perforin (PRF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Perforin in supernatant and buffered solutions.

    A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples. 

    This kit will recognize both endogenous and recombinant Human Perforin.

  • Tested applicationsSandwich ELISAmore details
  • PlatformMicroplate

Properties

  • FunctionPlays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • Involvement in diseaseFamilial hemophagocytic lymphohistiocytosis 2
  • Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • DomainThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • Post-translational
    modifications
    N-glycosylated.
  • Cellular localizationCytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • Alternative names
    • Cytolysin
    • FLH2
    • Lymphocyte pore-forming protein
    • P1
    • PERF_HUMAN
    • Perforin 1
    • Perforin-1
    • PFP
    • Pore forming protein
    • prf1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab46068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Perforin (PRF1) Human ELISA Kit images

  • Representative standard curve using ab46068

Protocols

References for Perforin (PRF1) Human ELISA Kit (ab46068)

This product has been referenced in:
  • Freeman LM  et al. Myeloma-induced alloreactive T cells arising in myeloma-infiltrated bones include double-positive CD8+CD4+ T cells: evidence from myeloma-bearing mouse model. J Immunol 187:3987-96 (2011). Read more (PubMed: 21908738) »

See 1 Publication for this product

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Thank you for your response.   I would like to clarify that the kit batch we currently have in stock does contain standards vials 3060 pg/vial.   The following standards batch (1820 pg/vial) will be included in the next kit batch production p...

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Thanks for your new enquiry regarding ab46068 and ab46115. The quantity provided in standard vials is batch specific and it may vary from one batch to another. Regarding Perforin ELISA Kit, standard batch contains 3060 pg/vial. However, the followi...

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Thank you for your enquiry. The standard for both kits is lyophilised, thus it is not possible to provide the concentration in the vial. However, there will be a batch number-specific information/instruction printed on the label of the vial. This w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"