Overview

  • Product name
  • Description
    Rabbit polyclonal to Pericentrin
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 175 and 225 of Human Pericentrin (NP_006022.3)

  • Positive control
    • HeLa Cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab84542 was affinity purified using an epitope specific to Pericentrin immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/500. Formaldehyde-Fixed and acetone-fixed cells.
    Permeabilization with Triton-X 100 is recommended.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
    • Tissue specificity
      Expressed in all tissues tested, including placenta, liver, kidney and thymus.
    • Involvement in disease
      Microcephalic osteodysplastic primordial dwarfism 2
    • Domain
      Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
    • Cellular localization
      Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • Centrosome Marker antibody
      • Ken antibody
      • Kendrin antibody
      • KIAA0402 antibody
      • MOPD2 antibody
      • PCN antibody
      • PCNT 2 antibody
      • PCNT antibody
      • PCNT B antibody
      • PCNT_HUMAN antibody
      • PCNT1 antibody
      • PCNT2 antibody
      • PCNTB antibody
      • PCTN2 antibody
      • Pericentrin 1 antibody
      • Pericentrin 2 antibody
      • Pericentrin 380 antibody
      • Pericentrin antibody
      • Pericentrin B antibody
      • Pericentrin-B antibody
      • SCKL4 antibody
      see all

    Anti-Pericentrin antibody images

    • ab84542 at 1/100 dilution staining Pericentrin in HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.
    • ab84542 at 1/500 dilution staining Pericentrin in asynchronous HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.
    • ab84542 at 1/500 dilution staining Pericentrin in asynchronous HeLa cells by Immunofluorescence, NBF-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.
    • ab84542 at 1/100 dilution staining Pericentrin in asynchronous HeLa cells by Immunofluorescence, Acetone-fixed. Detection: Red-fluorescent Alexa Fluor 594 goat antirabbit IgG.

    References for Anti-Pericentrin antibody (ab84542)

    ab84542 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab84542.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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