Anti-Pericentrin antibody - Centrosome Marker (ab4448)

Overview

  • Product nameAnti-Pericentrin antibody - Centrosome Marker
    See all Pericentrin primary antibodies
  • Description
    Rabbit polyclonal to Pericentrin - Centrosome Marker
  • SpecificityThis antibody should recognise both Pericentrin and Kendrin (also known as Pericentrin-2).
  • Tested applicationsSuitable for: ICC/IF, IHC-P, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, African Green Monkey
  • Immunogen

    The pericentrin clone used is 1.7 kb in size and is derived from within residues 100-600 of mouse pericentrin 1. It was expressed as a fusion protein. The corresponding amino acids are present in both pericentrin and kendrin (pericentrin-2) so this antibody is predicted to cross-react with both isoforms.

  • Positive control
    • ICC/IF: MCF7 and NIH3T3 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 0.1 - 0.5 µg/ml.
IHC-P 1/4500.
ICC Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.

Target

  • FunctionIntegral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • Tissue specificityExpressed in all tissues tested, including placenta, liver, kidney and thymus.
  • Involvement in diseaseMicrocephalic osteodysplastic primordial dwarfism 2
  • DomainComposed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • Cellular localizationCytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centrosome Marker antibody
    • Ken antibody
    • Kendrin antibody
    • KIAA0402 antibody
    • MOPD2 antibody
    • PCN antibody
    • PCNT 2 antibody
    • PCNT antibody
    • PCNT B antibody
    • PCNT_HUMAN antibody
    • PCNT1 antibody
    • PCNT2 antibody
    • PCNTB antibody
    • PCTN2 antibody
    • Pericentrin 1 antibody
    • Pericentrin 2 antibody
    • Pericentrin 380 antibody
    • Pericentrin antibody
    • Pericentrin B antibody
    • Pericentrin-B antibody
    • SCKL4 antibody
    see all

Anti-Pericentrin antibody - Centrosome Marker images

  • ICC/IF image of ab4448 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab4448, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab4448 stained NIH-3T3 cells. The cells were 100% methanol fixed for 5 minutes at -20°C and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • NIH3T3 cells were fixed in 100% methanol for 6 minutes at -20°C, washed 3 times in PBS then incubated with ab4448 (1/2000) for 1 hour at room temperature. The panel of images shows the nuclei stained with DAPI (blue), ab4448 staining is shown in green. 100x magnification.

  • Image courtesy of Human Protein Atlas

        Paraffin embedded human kidney tissue sections were incubated with ab4448 (1/4500 dilution) for 30 minutes at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab4448 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org

  • IF staining of pericentrin in MCF7 cells (human).

    The top panel is an interphase cell showing centrosome staining.  The bottom panel shows a mitotic cell with spindle pole staining.  ab4448 was used at 1/500, but also works at higher dilutions (1/1000-1/2000).

    Top panel - 630X magnification; Bottom panel -1000X magnification. The secondary antibody was Alexa488 anti-rabbit.

  • ICC/IF image of ab4448 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4448, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Pericentrin antibody - Centrosome Marker (ab4448)

This product has been referenced in:
  • Denu RA  et al. Centrosome amplification induces high grade features and is prognostic of worse outcomes in breast cancer. BMC Cancer 16:47 (2016). IHC . Read more (PubMed: 26832928) »
  • Kushner EJ  et al. Excess Centrosomes Perturb Dynamic Endothelial Cell Repolarization During Blood Vessel Formation. Mol Biol Cell N/A:N/A (2016). IHC . Read more (PubMed: 27099371) »

See all 129 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Gel Running Conditions Reduced Denaturing (4-12 Bis tris)
Loading amount 5e+006 cells
Specification 293T
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Mar 18 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HT1080)
Permeabilization Yes - 0.5% Triton X100
Specification HT1080
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 18°C
Fixative Paraformaldehyde
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Submitted Mar 18 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (mouse prostate and mammary epithelial)
Specification mouse prostate and mammary epithelial
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
Fixative try both PFA and methanol
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Submitted Aug 13 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (MCF10A Breast Cancer Cell Line)
Specification MCF10A Breast Cancer Cell Line
Permeabilization No
Fixative 1% Gluteraldehyde
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Submitted May 13 2014

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (breast cell line)
Specification breast cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Fixative Methanol
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Submitted Mar 11 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
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Submitted Jan 03 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C
Sample Monkey Cell (COS)
Specification COS
Permeabilization Yes - 0.1% Triton
Fixative Paraformaldehyde
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Submitted Jan 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - 0.1% Triton X100
Fixative Paraformaldehyde
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Submitted Sep 17 2013

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
Sample Monkey Cell (cos-7)
Specification cos-7
Permeabilization Yes - 0.1% Triton X100
Fixative Paraformaldehyde
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Submitted Sep 17 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step 2% BSA + 5% NGS as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 25°C
Sample Human Cell (Human umbilical vein endothelial cell)
Specification Human umbilical vein endothelial cell
Permeabilization Yes - 0.1% saponin
Fixative Paraformaldehyde
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Mr. Josh Meisner

Verified customer

Submitted May 28 2013

1-10 of 31 Abreviews or Q&A

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