Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144)

Overview

  • Product name
    Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker
    See all Pericentrin primary antibodies
  • Description
    Mouse monoclonal [mAbcam 28144] to Pericentrin - Centrosome Marker
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human, Chinese hamster
    Does not react with: Mouse
  • Immunogen

    The pericentrin clone used is 1.7 kb in size and is derived from within residues 100-600 of mouse pericentrin 1. It was expressed as a fusion protein. The corresponding amino acids are present in both pericentrin and kendrin (pericentrin-2) so this antibody is predicted to cross-react with both isoforms.

  • General notes

    This antibody clone is manufactured by Abcam.

    We can conjugate this antibody to FITC for you (please see ab150205 for details).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Contains 0.4M Arginine
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    mAbcam 28144
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab28144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. Plays a role, together with DISC1, in the microtubule network formation. Is an integral component of the pericentriolar material (PCM). May play an important role in preventing premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome.
  • Tissue specificity
    Expressed in all tissues tested, including placenta, liver, kidney and thymus.
  • Involvement in disease
    Microcephalic osteodysplastic primordial dwarfism 2
  • Domain
    Composed of a coiled-coil central region flanked by non-helical N- and C-terminals.
  • Cellular localization
    Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Centrosomal at all stages of the cell cycle. Remains associated with centrosomes following microtubule depolymerization. Colocalized with DISC1 at the centrosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Centrosome Marker antibody
    • Ken antibody
    • Kendrin antibody
    • KIAA0402 antibody
    • MOPD2 antibody
    • PCN antibody
    • PCNT 2 antibody
    • PCNT antibody
    • PCNT B antibody
    • PCNT_HUMAN antibody
    • PCNT1 antibody
    • PCNT2 antibody
    • PCNTB antibody
    • PCTN2 antibody
    • Pericentrin 1 antibody
    • Pericentrin 2 antibody
    • Pericentrin 380 antibody
    • Pericentrin antibody
    • Pericentrin B antibody
    • Pericentrin-B antibody
    • SCKL4 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence analysis of Y79 cells labeling Pericentrin with ab28144 (red). Cells were fixed for 10 minutes at room temperature in 4% paraformaldehyde. Cells were then permeabilized using 0.2% Triton/TBS and blocked with 5% NGS in 0.1% BSA/TBS-Tween. The cells were then incubated overnight at 4°C with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) at 1/1000 diluted in 0.1% BSA/TBS-Tween containing 1% NGS. Cells were then stained for Hoechst (blue) and alpha-tubulin (green).

  • ICC/IF image of ab28144 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab28144, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

     

  • Immunocytochemistry/ Immunofluorescence analysis of A549 lung cells labeling Pericentrin with ab28144 at 1/100 dilution. Cells were fixed with formaldehyde and permeabilized with Trixton X-100. The cells were blocked with 10% serum for 30 minutes at 20°C, followed by incubation with ab28144 in PBS 10% FCS for 1 hour at 20°C. A polyclonal goat anti-mouse Alexa Fluor® 594 secondary antibody was used at 1/300 dilution.

     

    See Abreview

  • Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Pericentrin with ab28144 at 1/200 dilution. Cells were fixed with methanol, followed by incubation with Anti-Pericentrin antibody [mAbcam 28144] - Centrosome Marker (ab28144) in PBS for 1 hour at 22°C. A polyclonal goat anti-mouse Cy3® secondary antibody was used at 1/200 dilution. Nuclear counterstained with DAPI.

    See Abreview

  • Overlay histogram showing HeLa cells stained with ab28144 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab28144, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References

This product has been referenced in:
  • Li J  et al. The kinetochore-dependent and -independent formation of the CDC20-MAD2 complex and its functions in HeLa cells. Sci Rep 7:41072 (2017). Read more (PubMed: 28112196) »
  • Li X  et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis. Nat Commun 8:14866 (2017). ICC/IF ; Human . Read more (PubMed: 28361952) »

See all 35 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT1080)
Permeabilization
Yes - 0.2% Triton
Specification
HT1080
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 25 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Permeabilization
Yes - 02% triton
Specification
HeLa
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 25 2017

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (HCT116)
Specification
HCT116
Permeabilization
Yes - when Paraformaldehyde
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Oct 17 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Young adult mouse colonic epithelium (YAMC) cells)
Permeabilization
No
Specification
Young adult mouse colonic epithelium (YAMC) cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Methanol
Username

Abcam user community

Verified customer

Submitted Sep 25 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
No
Username

Dr. Kirk Mcmanus

Verified customer

Submitted Oct 14 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cor1 cells)
Specification
Cor1 cells
Fixative
Formaldehyde
Permeabilization
Yes - TBS/BSA/azide/0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Aug 26 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549 lung cells)
Specification
A549 lung cells
Fixative
Formaldehyde
Permeabilization
Yes - tx 100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 11 2009

Unfortunately, we have not determined the IgG subclass for product ab28144.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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