Overview

  • Product name
  • Description
    Rabbit polyclonal to Perilipin-1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human Perilipin-1 aa 100-200 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O60240

  • Positive control
    • WB: Mouse Brown Adipose (BAT) P7 and P0 tissue lysates. IHC-P: Human breast adipose tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab126639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificity
    Adipocytes.
  • Sequence similarities
    Belongs to the perilipin family.
  • Post-translational
    modifications
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localization
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Images

  • All lanes : Anti-Perilipin-1 antibody (ab126639) at 1 µg/ml

    Lane 1 : P0 Mouse Brown Adipose Tissue Mouse Tissue Lysate
    Lane 2 : P7 Rat Brown Adipose Tissue Rat Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 62 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    The predicted molecular weight of Perilipin-1 is 56 kDa (SwissProt), however we expect to observe a banding pattern around 62 kDa.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab126639 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • IHC image of Perilipin-1 staining in human breast adipose formalin fixed paraffin embedded tissue, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab126639, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

ab126639 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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