Overview

  • Product name
  • Description
    Rabbit polyclonal to Perilipin-1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Rat Perilipin-1 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab109636)

  • Positive control
    • WB: Mouse brown adipose tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 56 kDa).

Target

  • Function
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificity
    Adipocytes.
  • Sequence similarities
    Belongs to the perilipin family.
  • Post-translational
    modifications
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localization
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Images

  • Anti-Perilipin-1 antibody (ab87129) at 1 µg/ml + Adult Mouse Brown Adipose Tissue Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    The predicted molecular weight of Perilipin-1 is 56 kDa (SwissProt), however we expect to observe a banding pattern at 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

ab87129 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - other (Duodenum Cytosolic Lipid Dropets)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
30 µg
Specification
Duodenum Cytosolic Lipid Dropets
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted May 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up