Overview

  • Product name
  • Description
    Rabbit polyclonal to Perilipin-1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Rat Perilipin-1 aa 1-100 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab109636)

  • Positive control
    • WB: Mouse brown adipose tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab87129 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 56 kDa).

Target

  • Function
    Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificity
    Adipocytes.
  • Sequence similarities
    Belongs to the perilipin family.
  • Post-translational
    modifications
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localization
    Lipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin A antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Anti-Perilipin-1 antibody images

  • Anti-Perilipin-1 antibody (ab87129) at 1 µg/ml + Adult Mouse Brown Adipose Tissue Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,47 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    The predicted molecular weight of Perilipin-1 is 56 kDa (SwissProt), however we expect to observe a banding pattern at 65 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References for Anti-Perilipin-1 antibody (ab87129)

ab87129 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Tissue lysate - other (Duodenum Cytosolic Lipid Dropets)
Gel Running Conditions
Reduced Denaturing (12.5%)
Loading amount
30 µg
Specification
Duodenum Cytosolic Lipid Dropets
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted May 15 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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