Anti-Perilipin 3 antibody (ab118605)

Overview

  • Product nameAnti-Perilipin 3 antibody
    See all Perilipin 3 primary antibodies
  • Description
    Goat polyclonal to Perilipin 3
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide:

    C-NQKTPQDAEKDPAK

    , corresponding to internal sequence amino acids 309-322 of Mouse M6PRBP1 (Q9DBG5).

  • Positive control
    • Mouse Spleen, Mouse Testis, Rat Skin lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab118605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 47 kDa.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionRequired for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
  • Sequence similaritiesBelongs to the perilipin family.
  • Cellular localizationCytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa mannose 6-phosphate receptor-binding protein antibody
    • 47 kDa MPR binding protein antibody
    • 47 kDa MPR-binding protein antibody
    • Cargo selection protein TIP47 antibody
    • M6PRBP 1 antibody
    • M6PRBP1 antibody
    • Mannose 6 phosphate receptor binding protein 1 antibody
    • Mannose-6-phosphate receptor-binding protein 1 antibody
    • MGC11117 antibody
    • MGC2012 antibody
    • MPR binding protein 47 kDa antibody
    • Perilipin 3 antibody
    • Perilipin-3 antibody
    • Perilipin3 antibody
    • Placental protein 17 antibody
    • PLIN3 antibody
    • PLIN3_HUMAN antibody
    • pp17 antibody
    • Tail interacting protein 47 kD antibody
    • TIP47 antibody
    see all

Anti-Perilipin 3 antibody images

  • ab118605 at 5 µg/ml staining M6PRBP1 in Mouse Spleen by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • ab118605 at 5 µg/ml staining M6PRBP1 in Mouse Testis by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue.
  • Anti-Perilipin 3 antibody (ab118605) at 0.1 µg/ml + Rat Skin lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 47 kDa

References for Anti-Perilipin 3 antibody (ab118605)

ab118605 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton X-100
Specification Brain
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Jun 07 2016

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Tissue lysate - whole (mouse skin)
Specification mouse skin
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Feb 09 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"