Overview

  • Product nameAnti-Perilipin 3 antibody
    See all Perilipin 3 primary antibodies
  • Description
    Rabbit polyclonal to Perilipin 3
  • Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    A 14 amino acid peptide from near the carboxy terminus of human M6PRBP1

  • Positive control
    • Daudi cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab47638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionRequired for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
  • Sequence similaritiesBelongs to the perilipin family.
  • Cellular localizationCytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa mannose 6-phosphate receptor-binding protein antibody
    • 47 kDa MPR binding protein antibody
    • 47 kDa MPR-binding protein antibody
    • Cargo selection protein TIP47 antibody
    • M6PRBP 1 antibody
    • M6PRBP1 antibody
    • Mannose 6 phosphate receptor binding protein 1 antibody
    • Mannose-6-phosphate receptor-binding protein 1 antibody
    • MGC11117 antibody
    • MGC2012 antibody
    • MPR binding protein 47 kDa antibody
    • Perilipin 3 antibody
    • Perilipin-3 antibody
    • Perilipin3 antibody
    • Placental protein 17 antibody
    • PLIN3 antibody
    • PLIN3_HUMAN antibody
    • pp17 antibody
    • Tail interacting protein 47 kD antibody
    • TIP47 antibody
    see all

Anti-Perilipin 3 antibody images

  • ab47638 staining Perilipin 3 in Human HUVEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, pH 7.4 for 5 minutes at room temperature and blocked with 5% BSA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour. A CF488A-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Lane 1 : Anti-Perilipin 3 antibody (ab47638) at 0.5 µg/ml
    Lane 2 : Anti-Perilipin 3 antibody (ab47638) at 1 µg/ml

    Lane 1 : Daudi cell lysate
    Lane 2 : Daudi cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab47638 at 10 µg/ml staining M6RBP1 in rat liver tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • Immunofluorescence of TIP47 in Rat Liver cells using ab47638 at 20 ug/ml.

References for Anti-Perilipin 3 antibody (ab47638)

ab47638 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (HUVEC)
Specification HUVEC
Permeabilization Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
Fixative Paraformaldehyde
Username

Dr. Dimitra Kalamida

Verified customer

Submitted Dec 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"