Overview

  • Product name
  • Description
    Rabbit polyclonal to Perilipin 3
  • Tested applications
    Suitable for: WB, IHC-P, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A 14 amino acid peptide from near the amino terminus of human M6PRBP1.

  • Positive control
    • EL4 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47639 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
IHC-P Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration. PubMed: 23593007

Target

  • Function
    Required for the transport of mannose 6-phosphate receptors (MPR) from endosomes to the trans-Golgi network.
  • Sequence similarities
    Belongs to the perilipin family.
  • Cellular localization
    Cytoplasm. Endosome membrane. Lipid droplet. Membrane associated on endosomes. Detected in the envelope and the core of lipid bodies and in lipid sails.
  • Information by UniProt
  • Database links
  • Alternative names
    • 47 kDa mannose 6-phosphate receptor-binding protein antibody
    • 47 kDa MPR binding protein antibody
    • 47 kDa MPR-binding protein antibody
    • Cargo selection protein TIP47 antibody
    • M6PRBP 1 antibody
    • M6PRBP1 antibody
    • Mannose 6 phosphate receptor binding protein 1 antibody
    • Mannose-6-phosphate receptor-binding protein 1 antibody
    • MGC11117 antibody
    • MGC2012 antibody
    • MPR binding protein 47 kDa antibody
    • Perilipin 3 antibody
    • Perilipin-3 antibody
    • Perilipin3 antibody
    • Placental protein 17 antibody
    • PLIN3 antibody
    • PLIN3_HUMAN antibody
    • pp17 antibody
    • Tail interacting protein 47 kD antibody
    • TIP47 antibody
    see all

Images

  • Lane 1 : Anti-Perilipin 3 antibody (ab47639) at 0.5 µg/ml
    Lane 2 : Anti-Perilipin 3 antibody (ab47639) at 1 µg/ml

    Lane 1 : EL4 cells
    Lane 2 : EL4 cells

    Lysates/proteins at 15 µg per lane.

    Secondary
    Anti-rabbit IgG HRP at 1:10000

    Predicted band size : 48 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • ab47639 at 2µg/ml staining M6PRBP1 in rat liver tissue by IHC

References

This product has been referenced in:
  • Vogt DA  et al. Lipid droplet-binding protein TIP47 regulates hepatitis C Virus RNA replication through interaction with the viral NS5A protein. PLoS Pathog 9:e1003302 (2013). WB, IP ; Human . Read more (PubMed: 23593007) »
  • Cabodevilla AG  et al. Cell survival during complete nutrient deprivation depends on lipid droplet-fueled ß-oxidation of fatty acids. J Biol Chem 288:27777-88 (2013). Read more (PubMed: 23940052) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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