Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab3526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Can be blocked with Perilipin A peptide (ab5009). By Western blot, this antibody detects an ~62 kDa protein representing Perilipin A from 3T3-L1 cell extract
IHC-FoFr Use at an assay dependent concentration. PubMed: 20049087
ICC/IF Use at an assay dependent concentration. PubMed: 20335253

Target

  • FunctionModulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness.
  • Tissue specificityAdipocytes.
  • Sequence similaritiesBelongs to the perilipin family.
  • Post-translational
    modifications
    Major cAMP-dependent protein kinase-substrate in adipocytes, also dephosphorylated by PP1. When phosphorylated, may be maximally sensitive to HSL and when unphosphorylated, may play a role in the inhibition of lipolysis, by acting as a barrier in lipid droplet.
  • Cellular localizationLipid droplet. Lipid droplet surface-associated.
  • Information by UniProt
  • Database links
  • Alternative names
    • Lipid droplet associated protein antibody
    • Lipid droplet-associated protein antibody
    • PERI antibody
    • Perilipin antibody
    • Perilipin-1 antibody
    • PerilipinA antibody
    • PLIN antibody
    • PLIN1 antibody
    • PLIN1_HUMAN antibody
    see all

Anti-Perilipin A antibody images

  • Western blot detection of Perilipin A in 3T3-L1 cell extract using ab3526.

  • Immunofluorescence analysis of Human preadipocytes, staining Perilipin A with ab3526. An AlexaFluor®568-conjugated anti-rabbit IgG was used as the secondary antibody.
  • ab3526 (4µg/ml) staining perilipin in Human breast adipose using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of adipocytes.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunohistochemical analysis of dog mammary adipose tissue, staining Perilipin A with ab3526.

    Tissue was fixed with formaldehyde and blocked with 1% blocking solution for 15 minutes at room temperature; antigen retrieval was by heat mediation in Tris-EDTA buffer (pH 9). Samples were incubated with primary antibody (1/200 in BSA in TBS) for 30 minutes. An undiluted HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

  • ab3526 staining Perilipin A in rat myofibers by Immunohistochemistry (PFA fixed).
    Freshly isolated single myofibers were collected in 0.5 ml DMEM, 5% HS in an Eppendorf tube. Fibers and satellite cells were fixed with 4% paraformaldehyde in phosphate buffered saline, rinsed in PBS and permeabilized with Triton X-100, 0.5% in PBS. After washing, fibers and/or cells were incubated with ab3526 at a 1/50 dilution overnight at 4°C or 1 hour at 37°C. Non specific interactions were blocked with 20% goat serum. They were then washed and incubated with labeled secondary antibodies for one hour at room temperature. Satellite cells were then mounted with fluorescent mounting medium plus DAPI 100 ng/ml. Fibers were collected and moved onto a polilysine microscope slide, and then mounted.

References for Anti-Perilipin A antibody (ab3526)

This product has been referenced in:
  • Zhao X  et al. Perilipin1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice. PLoS One 10:e0123738 (2015). Mouse . Read more (PubMed: 25855981) »
  • Gao Z  et al. P65 inactivation in adipocytes and macrophages attenuates adipose inflammatory response in lean but not in obese mice. Am J Physiol Endocrinol Metab 308:E496-505 (2015). WB ; Mouse . Read more (PubMed: 25564477) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Pig Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Buffer pH 9,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 10 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Other
Sample Cow Tissue sections (adipose)
Specification adipose
Permeabilization Yes - 30 min 1x PBS 0.5% tritron buffer
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 06 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (Mammary Adipose Tissue)
Specification Mammary Adipose Tissue
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 9 EDTA
Permeabilization No
Blocking step DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Nov 30 2012

Thank you for your reply.

Ypu are correct. The expiration is 27 March 2013.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit m...

Read More

I am very pleased to hear you would like to accept our offer and test ab3526 in Canine. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for Canine. The code is i...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (3T3-L1)
Specification 3T3-L1
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Nov 27 2006

Thank you for your enquiry. As stated on the datasheet, this antibody has only been tested for cross reactivity with mouse and human species. Therefore, we cannot guarantee that there will be no cross-reactivity with chicken. This however, does not mea...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"